Сафранин: Химические свойства и применение.

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| Name = Safranin
| ImageFile = Safranin Cl. svg
| ImageSize = 200px
| ImageName = Safranin
| ImageFile1 = Safranin 3D balls. png
| ImageSize1 = 200px
| ImageName1 = Safranin
| ImageFileL2 = Sample of Safranin O. jpg
| ImageSizeL2 = 130
| ImageCaptionL2 = Solid safranin
| ImageFileR2 = Safranin O in aqueous solution. jpg
| ImageSizeR2 = 130
| ImageCaptionR2 = Safranin in aqueous solution
| PIN = 3,7 Diamino 2,8 dimethyl 5 phenylphenazin 5 ium chloride
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|Section3=Chembox Hazards
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| GHSSignalWord = Danger mucin and mast cell granules. Safranin typically has the chemical structure shown at right (sometimes described as dimethyl safranin). There is also trimethyl safranin, which has an added methyl group in the ortho position (see Arene substitution pattern) of the lower ring. Both compounds behave essentially identically in biological staining applications, and most manufacturers of safranin do not distinguish between the two. Commercial safranin preparations often contain a blend of both types. Safranin is also used as redox indicator in analytical chemistry.

Safranines are the azonium compounds of symmetrical 2,8 dimethyl 3,7 diaminophenazine. They are obtained by the joint oxidation of one molecule of a para diamine with two molecules of a primary amine; by the condensation of para aminoazo compounds with primary amines, and by the action of para nitrosodialkylanilines with secondary bases such as diphenylmetaphenylenediamine. They are crystalline solids showing a characteristic green metallic lustre; they are readily soluble in water and dye red or violet. They are strong bases and form stable monacid salts. Their alcoholic solution shows a yellow red fluorescence. Phenosafranine is not very stable in the free state; its chloride forms green plates. It can be readily diazotized, and the diazonium salt when boiled with alcohol yields aposafranine or benzene induline, C18H12N3. Friedrich Kehrmann showed that aposafranine could be diazotized in the presence of cold concentrated sulfuric acid, and the diazonium salt on boiling with alcohol yielded phenylphenazonium salts. Aposafranone, C18H12N2O, is formed by heating aposafranine with concentrated hydrochloric acid. These three compounds are perhaps to be represented as ortho or as para quinones. The "safranine" of commerce is an ortho tolusafranine. The first aniline dye stuff to be prepared on a manufacturing scale was mauveine, which was obtained by Sir William Henry Perkin by heating crude aniline with potassium dichromate and sulfuric acid. Another well known safranin is phenosafranine (C. I. 50200, 3,7 diamino 5 phenylphenazinium chloride) widely used as a histological dye, photosensitizer and redox probe.