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The Children's Hospital of Philadelphia, also known by its acronym CHOP, is a children's hospital in Philadelphia, Pennsylvania. Its primary campus is located in the University City neighborhood of West Philadelphia on the campus of the University of Pennsylvania. The hospital has 594 beds It is one of the world's largest and oldest children's hospitals and the first hospital dedicated to the healthcare of children. CHOP has been ranked as the best children's hospital in the United States by U. S. News & World Report and Parents Magazine since 2020. it was ranked number one in the nation by U. S. News & World Report for three out of ten specialties. The hospital treats infants, children, teens, and young adults aged 0–21. The hospital also treats adults who would benefit from advanced pediatric care. The hospital is located next to the University of Pennsylvania, and its physicians serve as the pediatrics department of the Perelman School of Medicine at the University of Pennsylvania.

Construction adjacent to the second hospital began in 1913, and the first unit was opened in 1916, extending toward 18th and Bainbridge Streets. In 1919, the hospital became affiliated with the University of Pennsylvania School of Medicine. The affiliation became steadily closer over the next 17 years, with the Children's Hospital becoming identical to the pediatric department of the school of medicine, with most of the attending physicians appointed jointly to both institutions. In 1925, the hospital became affiliated with the Philadelphia Child Guidance Clinic. The whooping cough (pertussis) vaccine was first developed. The first formal allocation of funds to research was recorded in 1937. The first closed incubator for newborns was used. The nursing school was disbanded in 1945 and converted into an affiliate training center. A six story research building next to the hospital was dedicated in 1954. In 1962, under Dr. C. Everett Koop (later to be Surgeon General), the first neonatal intensive care unit (NICU) in the nation was opened, along with a new neonatal surgical unit. In 1965, the first home care program for children was established. A Clinical Research Center under the auspices of the National Institutes of Health was opened in 1965. In 1967, after years of lobbying hospital physicians and anesthesiologists, Dr. John Downes finally opened up a pediatric intensive care unit (PICU) at CHOP, the first of its kind in the United States. Downes was inspired by pediatric and adult intensive care units in Europe and wanted to open a state of the art unit in Philadelphia to care for the sickest of children. Before the creation of a PICU at CHOP, children who required advanced care were often cared for on the surgery wards and recovery rooms. Most of these children were cared for by anesthesiologists in the recovery room.

Construction of the new hospital at a new site on the west side of the Schuylkill River at 34th Street and Civic Center Boulevard, adjacent to the campus of the University of Pennsylvania, began in 1969, and the first building was opened in 1974. This present Children's Hospital complex occupies part of the site of the old Philadelphia General Hospital and Blockley Almshouse. A helicopter transport system for critically ill and injured children was inaugurated in 1973. Milestones and advances in pediatric care pioneered at CHOP include the first formal medical training in pediatrics, techniques for the correction of congenital heart malformations, incubators for newborn intensive care, home ventilator care, and vaccine development. In October 2006, Children's Hospital of Philadelphia's trauma center was one of the receiving hospitals' for victims of the West Nickel Mines School shooting, treating a few of the pediatric victims from the shooting who were medevaced to the hospital. The Children's Hospital of Philadelphia is in Philadelphia's University City neighborhood, and since 2001 has been undergoing a $1.5 billion expansion that has doubled the hospital's size, while also building more than of new research and outpatient facilities on a large, site south of the main hospital on Civic Center Boulevard. The South Campus expansion includes the eleven story Colket Translational Research Building, which provides lab space for the Center for Childhood Cancer Research and the Center for Cellular and Molecular Therapeutics. The new South Campus also includes an underground parking garage and an ambulatory care building with outpatient services. This South Campus expansion adjoins the University of Pennsylvania Health System's construction of the Perelman Center for Advanced Medicine and Roberts Proton Therapy Center. On July 1, 2015, Madeline Bell, previously CHOP's president and chief operating officer, became president and chief executive officer. She succeeded Steven M. Altschuler, MD, who retired after 15 years as CEO. The board of trustees of Children's Hospital made the announcement on May 14. Later that year, in October 2015, the expansion of the Brandywine Valley Specialty Care and Ambulatory Surgery Center was opened. This is a expansion. In May 2020 amidst the 2020 COVID 19 pandemic CHOP started offering virtual pediatric urgent care visits to all children aged 0–21 throughout Pennsylvania, New Jersey, New York, and Delaware regardless of if the child was a patient at the hospital or not.

CHOP has 594 beds, almost 40 percent of which are allocated to neonatal, cardiac, and pediatric intensive care. Each year the hospital admits more than 28,000 children and more than 1.167 million are seen in the emergency and outpatient departments.

The hospital has developed an expansion plan that includes four buildings along Schuylkill Avenue, on the east side of the Schuylkill River. The first building, a 375 feet (114 m) tower called the Roberts Center for Pediatric Research, or the CHOP Research Tower, was built between 2015 and 2017 on the 700 block of Schuylkill Avenue, with an address of 2716 South Street.

Children's Seashore House was founded in 1872 near Atlantic City, New Jersey, as a place for children to receive rehabilitation treatment. The hospital moved to its current location next to CHOP in 1990, and was acquired by CHOP in 1998, whereas of which the hospital has 45 beds. It currently provides inpatient and outpatient care for children with developmental disabilities and chronic illnesses.

In 2015, the Buerger Center for Advanced Pediatric Care opened and moved most outpatient services into the building. The addition is a 12 story, building with a five story, 1500 space underground parking garage directly attached. Composed of stacked forms and a selection of primary colors, the twelve story building and six story wing offer an interactive setting for treatment.

Pediatric care to the Penn Medicine Princeton Medical Center (PMC) is provided by doctors from the Children's Hospital of Philadelphia on PMC's inpatient pediatric wards, pediatric emergency department, and pediatric specialty care center. Adjacent to the medical center is the Children's Hospital of Philadelphia Specialty Center that treats infants, children, adolescents, and young adults up to the age of 21.

In addition to all of their outpatient centers, hospitals and primary care offices, CHOP also maintains an affiliation with the New Jersey based Saint Peter's University Hospital. All pediatric cardiac care at The Children's Hospital at Saint Peters is conducted from physicians affiliated with CHOP and complex cases are usually transferred to the hospital.

CHOP has an Adolescent & Young Adult Oncology Program for adolescents and young adults up to 30 to have treatment for their cancers.

Seacrest Studios (formerly known as The Voice) at Children's Hospital of Philadelphia is a closed circuit radio station and multimedia center. The studio, located in the main lobby, provides young patients within The Children's Hospital of Philadelphia community with an outlet to engage in activities related to radio, TV and new media, ranging from broadcasting like a disc jockey and playing their favorite songs to watching live artists perform and interviewing celebrities. The mission of Children's Hospital of Philadelphia's Media Programs and Seacrest Studios is building broadcast media centers, named Seacrest Studios, within the hospital to help in the healing process. Patients have access to radio, television and new media. Seacrest Studios opened in July 2011 and is the second media center to open after the completion of the first center November 2010 at Children's Healthcare of Atlanta in Georgia. Seacrest chose Children's Hospital of Philadelphia because it is a center of pediatric research and is focused on developing programs which enrich each patient's emotional well being. Seacrest Studios gives children the opportunity to conduct interviews with celebrities and watch live performances. The following Celebrities were guests of Seacrest Studios: Selena Gomez (who was also named as ambassador to the foundation in April 2012), CeeLo Green, Carrie Underwood, The Fray, Adam Levine, 5 Seconds of Summer, Taylor Swift, Julianne Hough, Jason Derulo, the cast of The Maze Runner, Hot Chelle Rae, Philadelphia Mayor Michael Nutter, Florida Georgia Line, R5, Austin Mahone and Rixton.

The Philadelphia Ronald McDonald House was the first of the Ronald McDonald House Charities and now stands at 39th and Chestnut Streets in West Philadelphia. The House opened with the help of Philadelphia Eagles manager Jimmy Murray and The Children's Hospital of Philadelphia's Pediatric Oncologists Dr. Audrey Evans and Dr. Milton 'Mickey' Donaldson. In the early 1970s, Evans and Donaldson saw families spending night after night in the hospital while their children received medical care. She knew there had to be a better way and envisioned a house where families could stay. At the same time, the Philadelphia Eagles were fundraising in support of player Fred Hill's daughter, Kim, who was battling leukemia. In 2006, the first Ronald McDonald Family Room was opened at The Children's Hospital of Philadelphia in the Oncology Unit.

In addition to its clinical facilities and programs, CHOP supports multiple research programs and dedicated core facilities. Its first laboratory opened in 1922, and CHOP scientists' contributions include a gene therapy for Leber congenital amaurosis (Luxturna), antiviral vaccines like the aforementioned pertussis vaccine, and a new model for delivering gene therapy treatments for blood disorders. CHOP has research groups working on childhood cancer, autism, mitochondrial disorders, and many other diseases and conditions, and keeps expanding to include new laboratories and centers (like the Center for Craniofacial Innovation, opened in 2022 with Eric Liao as the director).

The Children's Hospital of Philadelphia has consistently been ranked among the best hospitals for children by U. S. News & World Report. A detailed ranking of pediatric facilities in the United States is printed in the publication's first stand alone "America's Best Children's Hospitals" issue. In 2020, Children's Hospital of Philadelphia was ranked No. 2 nationwide and No. 1 in Pennsylvania in the U. S. News & World Report: Best Children's Hospital Ratings. In 2021 the hospital was ranked as the No. 2 best children's hospital in the United States by U. S. News & World Report on the publications' honor roll list. +U. S. News & World Report Rankings for Children's Hospital of PhiladelphiaSpecialtyRank (In the U. S.)Score (Out of 100)Neonatology391.7Pediatric Cancer794.7Pediatric Cardiology & Heart Surgery780.0Pediatric Diabetes & Endocrinology1100.0Pediatric Gastroenterology & GI Surgery1100.0Pediatric Nephrology496.2Pediatric Neurology & Neurosurgery298.6Pediatric Orthopedics395.4Pediatric Pulmonology & Lung Surgery298.2Pediatric Urology296.5