РНК-ның түрлі пре-мРН-дарынан экзондардың қосылуы: транс-түю процесі

Maturation process joining exons from different pre mRNAs into a mature mRNA
Trans splicing is a special form of RNA processing where exons from two different primary RNA transcripts are joined end to end and ligated. It is usually found in eukaryotes and mediated by the spliceosome, although some bacteria and archaea also have "half genes" for tRNAs.

Whereas "normal" (cis )splicing processes a single molecule, trans splicing generates a single RNA transcript from multiple separate pre mRNAs. This phenomenon can be exploited for molecular therapy to address mutated gene products. Genic trans splicing allows variability in RNA diversity and increases proteome complexity.

Spliced leader (SL) trans splicing is used by certain microorganisms, notably protists of the Kinetoplastea class to express genes. In these organisms, a capped splice leader RNA is transcribed, and simultaneously, genes are transcribed in long polycistrons. The capped splice leader is trans spliced onto each gene to generate monocistronic capped and polyadenylated transcripts. These early diverging eukaryotes use few introns, and the spliceosome they possess show some unusual variations in their structure assembly. They also possess multiple eIF4E isoforms with specialized roles in capping. The spliced leader sequence is highly conserved in lower species that undergo trans splicing. Such as trypanosomes. While the spliced leader's role is not known in the cell, it's thought to be involved in translation initiation. In C. elegans, the splicing of the sequence leader occurs close to the initiation codon. Some scientists also suggest the sequence is required for cell viability. In Ascaris, the spliced leader sequence is needed to the RNA gene can be transcribed. The Spliced leader sequence may be responsible for initiation, mRNA localization, and translation initiation or inhibition. In the tunicate Ciona intestinalis, the extent of SL trans splicing is better described by a quantitative view recognising frequently and infrequently trans spliced genes rather than a binary and conventional categorisation of trans spliced versus non trans spliced genes. The SL trans splicing functions in the resolution of polycistronic transcripts of operons into individual 5' capped mRNAs. This processing is achieved when the outrons are trans spliced to unpaired, downstream acceptor sites adjacent to cistron open reading frames.

Trans splicing is characterized by the joining of two separate exons transcribed RNAs. The signal for this splicing is the outron at the 5’ end of the mRNA, in the absence of a functional 5’ splice site upstream. When the 5’ outron in spliced, the 5’ splice site of the spliced leader RNA is branched to the outron and forms an intermediate. This step results in a free spliced leader exon. The exon is then spliced to the first exon on the pre mRNA and the intermediate is released. Trans splicing differs from cis splicing in that there is no 5' splice site on the pre mRNA. Instead the 5' splice site is provided by the SL sequence.

Alternative trans splicing includes intragenic trans splicing and intergenic trans splicing. Intragenic trans splicing involves duplication of exons in the pre mRNA. Intergenic trans splicing is characterized by the splicing together of exons formed form the pre mRNA of two different genes, resulting in trans genic mRNA.