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Process whereby cells acquire the properties of cancer

Malignant transformation is the process by which cells acquire the properties of cancer. This may occur as a primary process in normal tissue, or secondarily as malignant degeneration of a previously existing benign tumor.

There are many causes of primary malignant transformation, or tumorigenesis. Most human cancers in the United States are caused by external factors, and these factors are largely avoidable. These factors were summarized by Doll and Peto in 1981, Feeding the Western diet for two weeks to Native Africans increased their secondary bile acids, including carcinogenic deoxycholic acid, by 400%, and also changed the colonic microbiota. indicates that differences in human colonic microbiota play an important role in the progression of colon cancer.

A second example, relating a dietary component to a cancer, is illustrated by lung cancer. Two large population based studies were performed, one in Italy and one in the United States. In Italy, the study population consisted of two cohorts: the first, 1721 individuals diagnosed with lung cancer and no severe disease, and the second, 1918 control individuals with absence of lung cancer history or any advanced diseases. All individuals filled out a food frequency questionnaire including consumption of walnuts, hazelnuts, almonds, and peanuts, and indicating smoking status. In the United States, 495,785 members of AARP were questioned on consumption of peanuts, walnuts, seeds, or other nuts in addition to other foods and smoking status. In this U. S. study 18,533 incident lung cancer cases were identified during up to 16 years of follow up. Overall, individuals in the highest quintile of frequency of nut consumption had a 26% lower risk of lung cancer in the Italian study and a 14% lower risk of lung cancer in the U. S. study. Similar results were obtained among individuals who were smokers.

The most important chemical compounds in smoked tobacco that are carcinogenic are those that produce DNA damage since such damage appears to be the primary underlying cause of cancer. Cunningham et al. combined the microgram weight of the compound in the smoke of one cigarette with the known genotoxic effect per microgram to identify the most carcinogenic compounds in cigarette smoke. These compounds and their genotoxic effects are listed in the article Cigarette. The top three compounds are acrolein, formaldehyde and acrylonitrile, all known carcinogens.

In 2002 the World Health Organizations International Agency for Research on Cancer estimated that 11.9% of human cancers are caused by one of seven viruses (see Oncovirus overview table). These are Epstein Barr virus (EBV or HHV4); Kaposi's sarcoma associated herpesvirus (KSHV or HHV8); Hepatitis B and Hepatitis C viruses (HBV and HCV); Human T lymphotrophic virus 1 (HTLV 1); Merkel cell polyomavirus (MCPyV); and a group of alpha Human papillomaviruses (HPVs).

In 1995 epidemiologic evidence indicated that Helicobacter pylori infection increases the risk for gastric carcinoma. More recently, experimental evidence showed that infection with Helicobacter pylori cagA positive bacterial strains results in severe degrees of inflammation and oxidative DNA damage, leading to progression to gastric cancer.

Perera et al. referred to a number of articles pointing to roles of bacteria in other cancers. They pointed to single studies on the role of Chlamydia trachomatis in cervical cancer, Salmonella typhi in gallbladder cancer, and both Bacteroides fragilis and Fusobacterium nucleatum in colon cancer. Meurman has recently summarized evidence connecting oral microbiota with carcinogenesis. Although suggestive, these studies need further confirmation.

One underlying commonality in cancers is genetic mutation, acquired either by inheritance, or, more commonly, by mutations in one's somatic DNA over time. The mutations considered important in cancers are those that alter protein coding genes (the exome). As Vogelstein et al. point out, a typical tumor contains two to eight exome "driver gene" mutations, and a larger number of exome mutations that are "passengers" that confer no selective growth advantage. Cancers also generally have genome instability, that includes a high frequency of mutations in the noncoding DNA that makes up about 98% of the human genome. The average number of DNA sequence mutations in the entire genome of breast cancer tissue is about 20,000. In an average melanoma (where melanomas have a higher exome mutation frequency

A second underlying commonality in cancers is altered epigenetic regulation of transcription. In cancers, loss of gene expression occurs about 10 times more frequently by epigenetic transcription silencing (caused, for example, by promoter hypermethylation of CpG islands) than by mutations. As Vogelstein et al. Such methylation turns off expression of a gene as completely as a mutation would. Around 60–70% of human genes have a CpG island in their promoter region. In colon cancers, in addition to hypermethylated genes, several hundred other genes have hypomethylated (under methylated) promoters, thereby causing these genes to be turned on when they ordinarily would be turned off. Epigenetic silencing or epigenetic over expression of miRNA genes, caused by aberrant DNA methylation of the promoter regions controlling their expression, is a frequent event in cancer cells. Almost one third of miRNA promoters active in normal mammary cells were found to be hypermethylated in breast cancer cells, and that is a several fold greater proportion of promoters with altered methylation than is usually observed for protein coding genes. Other microRNA promoters are hypomethylated in breast cancers, and, as a result, these microRNAs are over expressed. Several of these over expressed microRNAs have a major influence in progression to breast cancer. BRCA1 is normally expressed in the cells of breast and other tissue, where it helps repair damaged DNA, or destroy cells if DNA cannot be repaired. BRCA1 is involved in the repair of chromosomal damage with an important role in the error free repair of DNA double strand breaks. BRCA1 expression is reduced or undetectable in the majority of high grade, ductal breast cancers. Only about 3–8% of all women with breast cancer carry a mutation in BRCA1 or BRCA2. BRCA1 promoter hypermethylation was present in only 13% of unselected primary breast carcinomas. However, breast cancers were found to have an average of about 100 fold increase in miR 182, compared to normal breast tissue. In breast cancer cell lines, there is an inverse correlation of BRCA1 protein levels with miR 182 expression. Thus it appears that much of the reduction or absence of BRCA1 in high grade ductal breast cancers may be due to over expressed miR 182. In addition to miR 182, a pair of almost identical microRNAs, miR 146a and miR 146b 5p, also repress BRCA1 expression. These two microRNAs are over expressed in triple negative tumors and their over expression results in BRCA1 inactivation. Thus, miR 146a and/or miR 146b 5p may also contribute to reduced expression of BRCA1 in these triple negative breast cancers. Post transcriptional regulation by microRNA occurs either through translational silencing of the target mRNA or through degradation of the target mRNA, via complementary binding, mostly to specific sequences in the three prime untranslated region of the target gene's mRNA. The mechanism of translational silencing or degradation of target mRNA is implemented through the RNA induced silencing complex (RISC).

Silencing of a DNA repair gene by hypermethylation or other epigenetic alteration appears to be a frequent step in progression to cancer. As summarized in a review, promoter hypermethylation of DNA repair gene MGMT occurs in 93% of bladder cancers, 88% of stomach cancers, 74% of thyroid cancers, 40% 90% of colorectal cancers and 50% of brain cancers. In addition, promoter hypermethylation of DNA repair genes LIG4, NEIL1, ATM, MLH1 or FANCB occurs at frequencies of between 33% and 82% in one or more of head and neck cancers, non small cell lung cancers or non small cell lung cancer squamous cell carcinomas. Further, the article Werner syndrome ATP dependent helicase indicates the DNA repair gene WRN has a promoter that is often hypermethylated in a variety of cancers, with WRN hypermethylation occurring in 11% to 38% of colorectal, head and neck, stomach, prostate, breast, thyroid, non Hodgkin lymphoma, chondrosarcoma and osteosarcoma cancers. Such silencing likely acts similarly to a germ line mutation in a DNA repair gene, and predisposes the cell and its descendants to progression to cancer. Another review points out that when a gene necessary for DNA repair is epigenetically silenced, DNA repair would tend to be deficient and DNA damages can accumulate. Increased DNA damage can cause increased errors during DNA synthesis, leading to mutations that give rise to cancer.

The heavy metals cadmium, arsenic and nickel are all carcinogenic when present above certain levels. Cadmium is known to be carcinogenic, possibly due to reduction of DNA repair. Lei et al. evaluated five DNA repair genes in rats after exposure of the rats to low levels of cadmium. They found that cadmium caused repression of three of the DNA repair genes: XRCC1 needed for base excision repair, OGG1 needed for base excision repair, and ERCC1 needed for nucleotide excision repair. Repression of these genes was not due to methylation of their promoters. Arsenic carcinogenicity was reviewed by Bhattacharjee et al. Nickel compounds exhibit weak mutagenic activity, but they considerably alter the transcriptional landscape of the DNA of exposed individuals.

Malignant transformation of cells in a benign tumor may be detected by pathologic examination of tissues. Often the clinical signs and symptoms are suggestive of a malignant tumor. The physician, during the medical history examination, can find that there have been changes in size or patient sensation and, upon direct examination, that there has been a change in the lesion itself. Risk assessments can be done and are known for certain types of benign tumor which are known to undergo malignant transformation. One of the better known examples of this phenomenon is the progression of a nevus to melanoma.