Missouri Route 240

State highway in central Missouri

Business Loop 240 runs entirely on the old routing of Route 240. It begins at the Glasgow end of the bridge over the Missouri River. It heads north concurrent with Route 5 through Downtown Glasgow. After passing through downtown, Route 5 leaves, and Business 240 runs eastward to the new routing of Routes 5 and 240.

Alternate Route 240 in rural Howard County is technically a spur route that provides easy access from eastbound Route 240 to westbound U. S. Route 40, and vice versa, utilizing an old alignment of U. S. Route 40. The route runs northeast from U. S. Route 40 at the southeastern edge of the Davidsdale Conservation Area, then passes through some farmland. After running along the northwestern edge of a small pond, it intersects Howard County Road 435A, and immediately the shared intersection of Howard County Roads 442 and 435. Before reaching its northeastern terminus, the road curves east, where a connecting road leads to westbound Route 240, while the existing road curves to the southeast and ends at eastbound Route 240.

Route 240 Spur is a spur route from US 40/MO 240 that runs towards Rocheport, Missouri. Though cardinal direction signs read "East," it actually runs south from its parent route, taking the Lewis and Clark Trail with it. The road then curves to the southeast where it has a wye intersection with Howard County Road 440. Spur Route 240 officially enters Rocheport, just before crossing a bridge over Moniteau Creek where it also crosses the Howard Boone County Line, then ends at Route BB (Third Street), which takes the Lewis and Clark Trail with it, while Spur Route 240 continues south as Central Avenue which leads to a portion of the Katy Trail State Park.