Микрококк нуклеазасы: қасиеттері, қолданылуы және құрылымы

Class of enzymes

Micrococcal nuclease (, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T''', staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3' nucleotidohydrolase) is an endo exonuclease that preferentially digests single stranded nucleic acids. The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3' phosphates. The enzyme is also active against double stranded DNA and RNA and all sequences will be ultimately cleaved. Characteristics

The enzyme has a molecular weight of 16.9kDa. The pH optimum is reported as 9.2. The enzyme activity is strictly dependent on Ca2+ and the pH optimum varies according to Ca2+ concentration. The enzyme is therefore easily inactivated by EGTA. Sources

This enzyme is the extracellular nuclease of Staphylococcus aureus. Two strains, V8 and Foggi, yield almost identical enzymes. A common source is E. coli'' cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease).

The 3 dimensional structure of micrococcal nuclease (then called Staphyloccal nuclease) was solved very early in the history of protein crystallography, in 1969, deposited as now obsolete Protein Data Bank file 1SNS. Higher resolution, more recent crystal structures are available for the apo form as Protein Data Bank file 1SNO: and for the thymidine diphosphate inhibited form as Protein Data Bank file 3H6M: or 1SNC: As seen in the ribbon diagram above, the nuclease molecule has 3 long alpha helices and a 5 stranded, barrel shaped beta sheet, in an arrangement known as the OB fold (for oligonucleotide binding fold) as classified in the SCOP database.

CUT&RUN sequencing, antibody targeted controlled cleavage by micrococcal nuclease for transcriptomic profiling. Hydrolysis of nucleic acids in crude cell free extracts. Sequencing of RNA. Preparation of rabbit reticulocyte lysates. Studies of chromatin structure. Removal of nucleic acids from laboratory protein preparations allowing for protein folding and structure function studies. Research on the mechanisms of protein folding.