РНБ – РНК байланыстыратын ақуыздар және гендік экспрессияны реттеудегі ролі

Type of protein

RNA binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes. RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others. They are cytoplasmic and nuclear proteins. However, since most mature RNA is exported from the nucleus relatively quickly, most RBPs in the nucleus exist as complexes of protein and pre mRNA called heterogeneous ribonucleoprotein particles (hnRNPs). RBPs have crucial roles in various cellular processes such as: cellular function, transport and localization. They especially play a major role in post transcriptional control of RNAs, such as: splicing, polyadenylation, mRNA stabilization, mRNA localization and translation. Eukaryotic cells express diverse RBPs with unique RNA binding activity and protein–protein interaction. According to the Eukaryotic RBP Database (EuRBPDB), there are 2961 genes encoding RBPs in humans. During evolution, the diversity of RBPs greatly increased with the increase in the number of introns. Diversity enabled eukaryotic cells to utilize RNA exons in various arrangements, giving rise to a unique RNP (ribonucleoprotein) for each RNA. Although RBPs have a crucial role in post transcriptional regulation in gene expression, relatively few RBPs have been studied systematically. It has now become clear that RNA–RBP interactions play important roles in many biological processes among organisms.

Many RBPs have modular structures and are composed of multiple repeats of just a few specific basic domains that often have limited sequences. Different RBPs contain these sequences arranged in varying combinations. A specific protein's recognition of a specific RNA has evolved through the rearrangement of these few basic domains. Each basic domain recognizes RNA, but many of these proteins require multiple copies of one of the many common domains to function.

Alternative splicing is a mechanism by which different forms of mature mRNAs (messengers RNAs) are generated from the same gene. It is a regulatory mechanism by which variations in the incorporation of the exons into mRNA leads to the production of more than one related protein, thus expanding possible genomic outputs. RBPs function extensively in the regulation of this process. Some binding proteins such as neuronal specific RNA binding proteins, namely NOVA1, control the alternative splicing of a subset of hnRNA by recognizing and binding to a specific sequence in the RNA (YCAY where Y indicates pyrimidine, U or C). These proteins then recruit splicesomal proteins to this target site. SR proteins are also well known for their role in alternative splicing through the recruitment of snRNPs that form the splicesome, namely U1 snRNP and U2AF snRNP. However, RBPs are also part of the splicesome itself. The splicesome is a complex of snRNA and protein subunits and acts as the mechanical agent that removes introns and ligates the flanking exons.

The most extensively studied form of RNA editing involves the ADAR protein. This protein functions through post transcriptional modification of mRNA transcripts by changing the nucleotide content of the RNA. This is done through the conversion of adenosine to inosine in an enzymatic reaction catalyzed by ADAR. This process effectively changes the RNA sequence from that encoded by the genome and extends the diversity of the gene products. The majority of RNA editing occurs on non coding regions of RNA; however, some protein encoding RNA transcripts have been shown to be subject to editing resulting in a difference in their protein's amino acid sequence. An example of this is the glutamate receptor mRNA where glutamine is converted to arginine leading to a change in the functionality of the protein. More recent studies of FMRP bound RNAs present in microdissected dendrites of CA1 hippocampal neurons revealed no changes in localization in wild type versus FMRP null mouse brains.

Translational regulation provides a rapid mechanism to control gene expression. Rather than controlling gene expression at the transcriptional level, mRNA is already transcribed but the recruitment of ribosomes is controlled. This allows rapid generation of proteins when a signal activates translation. ZBP1 in addition to its role in the localization of B actin mRNA is also involved in the translational repression of beta actin mRNA by blocking translation initiation. ZBP1 must be removed from the mRNA to allow the ribosome to properly bind and translation to begin. Specific binding of the RNA binding proteins allow them to distinguish their targets and regulate a variety of cellular functions via control of the generation, maturation, and lifespan of the RNA transcript. This interaction begins during transcription as some RBPs remain bound to RNA until degradation whereas others only transiently bind to RNA to regulate RNA splicing, processing, transport, and localization. Cross linking immunoprecipitation (CLIP) methods are used to stringently identify direct RNA binding sites of RNA binding proteins in a variety of tissues and organisms. In this section, three classes of the most widely studied RNA binding domains (RNA recognition motif, double stranded RNA binding motif, zinc finger motif) will be discussed.

The RNA recognition motif, which is the most common RNA binding motif, is a small protein domain of 75–85 amino acids that forms a four stranded β sheet against the two α helices. This recognition motif exerts its role in numerous cellular functions, especially in mRNA/rRNA processing, splicing, translation regulation, RNA export, and RNA stability. Ten structures of an RRM have been identified through NMR spectroscopy and X ray crystallography. These structures illustrate the intricacy of protein–RNA recognition of RRM as it entails RNA–RNA and protein–protein interactions in addition to protein–RNA interactions. Despite their complexity, all ten structures have some common features. All RRMs' main protein surfaces' four stranded β sheet was found to interact with the RNA, which usually contacts two or three nucleotides in a specific manner. In addition, strong RNA binding affinity and specificity towards variation are achieved through an interaction between the inter domain linker and the RNA and between RRMs themselves. This plasticity of the RRM explains why RRM is the most abundant domain and why it plays an important role in various biological functions. Extensive research on the nematode C. elegans has identified RNA binding proteins as essential factors during germline and early embryonic development. Their specific function involves the development of somatic tissues (neurons, hypodermis, muscles and excretory cells) as well as providing timing cues for the developmental events. Nevertheless, it is exceptionally challenging to discover the mechanism behind RBPs' function in development due to the difficulty in identifying their RNA targets. This is because most RBPs usually have multiple RNA targets. However, it is indisputable that RBPs exert a critical control in regulating developmental pathways in a concerted manner.

In Drosophila melanogaster, Elav, Sxl and tra 2 are RNA binding protein encoding genes that are critical in the early sex determination and the maintenance of the somatic sexual state. These genes impose effects on the post transcriptional level by regulating sex specific splicing in Drosophila. Sxl exerts positive regulation of the feminizing gene tra to produce a functional tra mRNA in females. In C. elegans, RNA binding proteins including FOG 1, MOG 1/ 4/ 5 and RNP 4 regulate germline and somatic sex determination. Furthermore, several RBPs such as GLD 1, GLD 3, DAZ 1, PGL 1 and OMA 1/ 2 exert their regulatory functions during meiotic prophase progression, gametogenesis, and oocyte maturation. Other RNA binding proteins involved in dendrite formation are Pumilio and Nanos, FMRP, CPEB and Staufen 1

RBPs are emerging to play a crucial role in tumor development. Hundreds of RBPs are markedly dysregulated across human cancers and showed predominant downregulation in tumors related to normal tissues. ELAVL1(HuR), FXR1 and UHMK1. For some RBPs, the change in expression are related with Copy Number Variations (CNV), for example CNV gains of BYSL in colorectal cancer cells Some expression changes are cause due to protein affecting mutations on these RBPs for example NSUN6, ZC3H13, ELAC1, RBMS3, and ZGPAT, SF3B1, SRSF2, RBM10, U2AF1, SF3B1, PPRC1, RBMXL1, HNRNPCL1 etc. Several studies have related this change in expression of RBPs to aberrant alternative splicing in cancer.

As RNA binding proteins exert significant control over numerous cellular functions, they have been a popular area of investigation for many researchers. Due to its importance in the biological field, numerous discoveries regarding RNA binding proteins' potentials have been recently unveiled. RNA binding protein Sam68 controls the spatial and temporal compartmentalization of RNA metabolism to attain proper synaptic function in dendrites. Loss of Sam68 results in abnormal posttranscriptional regulation and ultimately leads to neurological disorders such as fragile X associated tremor/ataxia syndrome. Sam68 was found to interact with the mRNA encoding β actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Therefore, Sam68 plays a critical role in regulating synapse number via control of postsynaptic β actin mRNA metabolism. Neuron specific CELF family RNA binding protein UNC 75 specifically binds to the UUGUUGUGUUGU mRNA stretch via its three RNA recognition motifs for the exon 7a selection in C. elegans''' neuronal cells. As exon 7a is skipped due to its weak splice sites in non neuronal cells, UNC 75 was found to specifically activate splicing between exon 7a and exon 8 only in the neuronal cells. The cold inducible RNA binding protein CIRBP plays a role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. This research yielded potential implications for the association of disease states with inflammation. Serine arginine family of RNA binding protein Slr1 was found exert control on the polarized growth in Candida albicans. Slr1 mutations in mice results in decreased filamentation and reduces damage to epithelial and endothelial cells that leads to extended survival rate compared to the Slr1 wild type strains. Therefore, this research reveals that SR like protein Slr1 plays a role in instigating the hyphal formation and virulence in C. albicans''.