Soft X-ray microscopy

An X ray microscope uses electromagnetic radiation in the soft X ray band to produce images of very small objects. Unlike visible light, X rays do not reflect or refract easily, and they are invisible to the human eye. Therefore, the basic process of an X ray microscope is to expose film or use a charge coupled device (CCD) detector to detect X rays that pass through the specimen. It is a contrast imaging technology using the difference in absorption of soft X ray in the water window region (wavelength region: 2.34–4.4 nm, photon energy region: 280 – 530 eV) by the carbon atom (main element composing the living cell) and the oxygen atom (main element for water).

Early X ray microscopes by Paul Kirkpatrick and Albert Baez used grazing incidence reflective optics to focus the X rays, which grazed X rays off parabolic curved mirrors at a very high angle of incidence. An alternative method of focusing X rays is to use a tiny fresnel zone plate of concentric gold or nickel rings on a silicon dioxide substrate. Sir Lawrence Bragg produced some of the first usable X ray images with his apparatus in the late 1940s. In the 1950s Newberry produced a shadow X ray microscope which placed the specimen between the source and a target plate, this became the basis for the first commercial X ray microscopes from the General Electric Company.

The Advanced Light Source (ALS) in Berkeley, California, is home to XM 1 (http://www. cxro. lbl. gov/BL612/), a full field soft X ray microscope operated by the Center for X ray Optics and dedicated to various applications in modern nanoscience, such as nanomagnetic materials, environmental and materials sciences and biology. XM 1 uses an X ray lens to focus X rays on a CCD, in a manner similar to an optical microscope. XM 1 held the world record in spatial resolution with Fresnel zone plates down to 15 nm and is able to combine high spatial resolution with a sub 100ps time resolution to study e. g. ultrafast spin dynamics. In July 2012, a group at DESY claimed a record spatial resolution of 10 nm, by using the hard X ray scanning microscope at PETRA III. The ALS is also home to the world's first soft X ray microscope designed for biological and biomedical research. This new instrument, XM 2 was designed and built by scientists from the National Center for X ray Tomography. XM 2 is capable of producing 3 Dimensional tomograms of cells.

In contrast to synchrotron based soft X ray tomography, lab based soft X ray tomography developed by SiriusXT setups offer a compact and easily integratable solution for researchers within laboratory settings. These systems typically utilize laser driven X ray sources, with the brightness of the source dependent on the type of target and the power of the laser. Notably, the SXT 100 table top soft X ray microscopes represent the only commercially available options in
this domain. These microscopes enable the acquisition of soft X ray tomograms from cryogenically vitrified as well as room temperature samples, employing flat specimen holders such as standard transmission electron microscopy (TEM) grids or glass capillaries. The SXT 100 system stands out for its versatility, accommodating a variety of specimen formats. For instance, researchers can acquire tomograms on TEM grids, allowing for ±60 degrees range tilt series collected in 1 degree steps within a time frame ranging from under one hour to two hours. In the case of specimens imaged in glass capillaries, the full tilt tomography may take slightly longer. Resolutions in biological specimens, determined by Fourier Ring Correlations (1/2 Signal to Noise ratio), can achieve 55 nm for two hour tomograms, with the ability to resolve Siemens star lines and spaces as small as 25 nm. These table top soft X ray microscopes enhance the accessibility of high resolution soft X ray tomography beyond large scale facilities. Their integration into currently established correlative light and electron microscopy workflows bridges the resolution gap and significantly improves imaging throughput.

Sources of soft X rays suitable for microscopy, such as synchrotron radiation sources, have fairly low brightness of the required wavelengths, so an alternative method of image formation is scanning transmission soft X ray microscopy. Here the X rays are focused to a point and the sample is mechanically scanned through the produced focal spot. At each point the transmitted X rays are recorded with a detector such as a proportional counter or an avalanche photodiode. This type of Scanning Transmission X ray Microscope (STXM) was first developed by researchers at Stony Brook University and was employed at the National Synchrotron Light Source at Brookhaven National Laboratory. The resolution of X ray microscopy lies between that of the optical microscope and the electron microscope. It has an advantage over conventional electron microscopy in that it can view biological samples in their natural state. Electron microscopy is widely used to obtain images with nanometer level resolution but the relatively thick living cell cannot be observed as the sample has to be chemically fixed, dehydrated, embedded in resin, then sliced ultra thin. However, it should be mentioned that cryo electron microscopy allows the observation of biological specimens in their hydrated natural state, albeit embedded in water ice. Until now, resolutions of 30 nanometer are possible using the Fresnel zone plate lens which forms the image using the soft X rays emitted from a synchrotron. Recently, the use of soft X rays emitted from laser produced plasmas rather than synchrotron radiation is becoming more popular. Additionally, X rays cause fluorescence in most materials, and these emissions can be analyzed to determine the chemical elements of an imaged object. Another use is to generate diffraction patterns, a process used in X ray crystallography. By analyzing the internal reflections of a diffraction pattern (usually with a computer program), the three dimensional structure of a crystal can be determined down to the placement of individual atoms within its molecules. X ray microscopes are sometimes used for these analyses because the samples are too small to be analyzed in any other way.