Сигнальдық жолдардағы қаңқалық ақуыздардың ролі мен функциялары

In biology, scaffold proteins are crucial regulators of many key signalling pathways. Although scaffolds are not strictly defined in function, they are known to interact and/or bind with multiple members of a signalling pathway, tethering them into complexes. In such pathways, they regulate signal transduction and help localize pathway components (organized in complexes) to specific areas of the cell such as the plasma membrane, the cytoplasm, the nucleus, the Golgi, endosomes, and the mitochondria.

The first signaling scaffold protein discovered was the Ste5 protein from the yeast Saccharomyces cerevisiae. Three distinct domains of Ste5 were shown to associate with the protein kinases Ste11, Ste7, and Fus3 to form a multikinase complex.

Scaffold proteins act in at least four ways: tethering signaling components, localizing these components to specific areas of the cell, regulating signal transduction by coordinating positive and negative feedback signals, and insulating correct signaling proteins from competing proteins. Scaffolds may also be catalytic as interaction with signaling proteins may result in allosteric changes of these signaling components. Such changes may be able to enhance or inhibit the activation of these signaling proteins. An example is the Ste5 scaffold in the mitogen activated protein kinase (MAPK) pathway. Ste5 has been proposed to direct mating signaling through the Fus3 MAPK by catalytically unlocking this particular kinase for activation by its MAPKK Ste7.

Scaffolds localize the signaling reaction to a specific area in the cell, a process that could be important for the local production of signaling intermediates. A particular example of this process is the scaffold, A kinase anchor proteins (AKAPs), which target cyclic AMP dependent protein kinase (PKA) to various sites in the cell. This localization is able to locally regulate PKA and results in the local phosphorylation by PKA of its substrates.

Many hypotheses about how scaffolds coordinate positive and negative feedback come from engineered scaffolds and mathematical modeling. In three kinase signaling cascades, scaffolds bind all three kinases, enhancing kinase specificity and restricting signal amplification by limiting kinase phosphorylation to only one downstream target. These abilities may be related to stability of the interaction between the scaffold and the kinases, the basal phosphatase activity in the cell, scaffold location, and expression levels of the signaling components.

Scaffold ProteinsPathwayPotential FunctionsDescriptionKSR MAPK Assembly and localization of the RAS ERK pathway One of the best studied signaling pathways in biology is the RAS ERK pathway in which the RAS G protein activates the MAPKKK RAF, which activates the MAPKK MEK1 (MAPK/ERK kinase 1), which then activates the MAPK ERK. Several scaffold proteins have been identified to be involved in this pathway and other similar MAPK pathways. One such scaffold protein is KSR, which is the most probable equivalent of the well studied yeast MAPK scaffold protein Ste5. It is a positive regulator of the pathway and binds many proteins in the pathway, including all three kinases in the cascade. MEKK1 MAPK Assembly and localization of the death receptor signalosome Other scaffold proteins include B cell lymphoma 10 (BCL 10) and MEK kinase 1 (MEKK1), which have roles in the JUN N terminal kinase (JNK) pathway. BCL 10 MAPK Assembly and specificity of JNKAKAP PKA Pathways Coordination of phosphorylation by PKA onto downstream targets This family of proteins is only structurally related in their ability to bind the regulatory subunit of PKA but can otherwise bind a very diverse set of enzymes and substratesAHNAK 1 Calcium signaling Assembly and localization of calcium channels Calcium signaling is essential for the proper function of immune cells. Recent studies have shown that the scaffold protein, AHNAK1, is important for efficient calcium signaling and NFAT activation in T cells through its ability to properly localize calcium channels at the plasma membrane [14]. In non immune cells, AHNAK1 has also been shown to bind calcium channels with phospholipase Cγ (PLC γ) and PKC. Through this competition, the HOMER proteins are able to reduce NFAT activation, which also reduces the production of the IL 2 cytokine. Pellino Innate Immune Signaling Assembly of the TLR signalosome Evidence exists that Pellino proteins function as scaffold proteins in the important innate immune signaling pathway, the Toll like receptor (TLR) pathway. Much Pellino function is speculation; however, Pellino proteins can associate with IRAK1, TRAF6, and TAK1 following IL 1R activation, indicating that they may assemble and localize components of the TLR pathway near its receptor. NLRP Innate Immune Signaling Assembly of the inflammasome The NLR family is a highly conserved and large family of receptors involved in innate immunity. The NLRP (NLR family, pyrine domain containing) family of receptors function as scaffolds by assembling the inflammasome, a complex that leads to the secretion of pro inflammatory cytokines such as IL 18 and IL 1β. DLG1 T cell receptor signaling Assembly and localization of TCR signaling molecules, activation of p38 DLG1 is highly conserved in immune cells and is important for T cell activation in the periphery. It is recruited to the immunological synapse and links the ζ chain of the T cell receptor (TCR) to CBL, WASP, p38, LCK, VAV1, and ZAP70. This data suggests that DLG1 plays a role in linking TCR signaling machinery with cytoskeleton regulators and also suggests a role in alternatively activating the p38 pathway. However, it is unclear to whether DLG1 positively or negatively regulates T cell activation. Spinophilin Dendritic cell signaling Assembly of DC immunological synapse proteins Spinophilin is involved in dendritic cell function specifically in the formation of immunological synapses. Spinophilin is recruited to the synapse following dendritic cell contact with a T cell. This recruitment seems to be important because without spinophilin, dendritic cells cannot activate T cells in vitro or in vivo. How spinophilin facilitates antigen presentation in this case is still unknown though it is possible that spinophilin regulates the duration of cell contact in the synapse or regulates the recycling of co stimulatory molecules in the cell like MHC molecules. Coordination of negative feedback during protochlorophyllide biosynthesis. Assembly and localization of the pathway that turns the synthesis of highly toxic protochlorophyllide, a precursor of chlorophyll. Synthesis of protochlorophyllide must be strictly regulated as its conversion into chlorophyll requires light. FLU regulatory protein is located in thylakoid membrane and only contains several protein protein interaction sites without catalytic activity. Mutants lacking this protein overaccumulate protochlorophyllide in the darkness. The interaction partners are unknown. The protein underwent simplification during evolution.

Huntingtin protein co localizes with ATM repair protein at sites of DNA damage. Huntingtin is a scaffolding protein in the ATM oxidative DNA damage response complex. Huntington's disease is likely caused by the dysfunction of mutant huntingtin scaffold protein in DNA repair leading to increased oxidative DNA damage in metabolically active cells. RAD51 and DMC1 are recombinases that act during mammalian meiosis to mediate strand exchange during the repair of DNA double strand breaks by homologous recombination. Chromosome scaffold constituent proteins are also called scaffold protein. In enzymatic reaction Large multifunctional enzymes that performs a series or chain of reaction in a common pathway, sometimes called scaffold proteins. such as Pyruvate dehydrogenase. In molecule shape formation An enzyme or structural protein that holds several molecules together to hold them in proper spatial arrangement, such as Iron sulphur cluster scaffold proteins. Structural scaffold In cytoskeleton and ECM, the molecules provide mechanical scaffold. Such as type 4 collagen