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Concept in inferential statistics
In statistical hypothesis testing, a result has statistical significance when a result at least as "extreme" would be very infrequent if the null hypothesis were true. and the p value of a result, , is the probability of obtaining a result at least as extreme, given that the null hypothesis is true. The result is statistically significant, by the standards of the study, when The significance level for a study is chosen before data collection, and is typically set to 5% or much lower—depending on the field of study. In any experiment or observation that involves drawing a sample from a population, there is always the possibility that an observed effect would have occurred due to sampling error alone. But if the p value of an observed effect is less than (or equal to) the significance level, an investigator may conclude that the effect reflects the characteristics of the whole population,

This technique for testing the statistical significance of results was developed in the early 20th century. The term significance does not imply importance here, and the term statistical significance is not the same as research significance, theoretical significance, or practical significance. For example, the term clinical significance refers to the practical importance of a treatment effect.

Statistical significance dates to the 18th century, in the work of John Arbuthnot and Pierre Simon Laplace, who computed the p value for the human sex ratio at birth, assuming a null hypothesis of equal probability of male and female births; see for details. In 1925, Ronald Fisher advanced the idea of statistical hypothesis testing, which he called "tests of significance", in his publication Statistical Methods for Research Workers. Fisher suggested a probability of one in twenty (0.05) as a convenient cutoff level to reject the null hypothesis. In a 1933 paper, Jerzy Neyman and Egon Pearson called this cutoff the significance level, which they named They recommended that be set ahead of time, prior to any data collection. Despite his initial suggestion of 0.05 as a significance level, Fisher did not intend this cutoff value to be fixed. In his 1956 publication Statistical Methods and Scientific Inference, he recommended that significance levels be set according to specific circumstances. Confidence levels and confidence intervals were introduced by Neyman in 1937.

Statistical significance plays a pivotal role in statistical hypothesis testing. It is used to determine whether the null hypothesis should be rejected or retained. The null hypothesis is the hypothesis that no effect exists in the phenomenon being studied. For the null hypothesis to be rejected, an observed result has to be statistically significant, i. e. the observed p value is less than the pre specified significance level , or sometimes, Emily Campbell's
To determine whether a result is statistically significant, a researcher calculates a p value, which is the probability of observing an effect of the same magnitude or more extreme given that the null hypothesis is true. and a statistically significant result is one where the observed p value is less than (or equal to) 5%. When drawing data from a sample, this means that the rejection region comprises 5% of the sampling distribution. These 5% can be allocated to one side of the sampling distribution, as in a one tailed test, or partitioned to both sides of the distribution, as in a two tailed test, with each tail (or rejection region) containing 2.5% of the distribution. The use of a one tailed test is dependent on whether the research question or alternative hypothesis specifies a direction such as whether a group of objects is heavier or the performance of students on an assessment is better. A two tailed test may still be used but it will be less powerful than a one tailed test, because the rejection region for a one tailed test is concentrated on one end of the null distribution and is twice the size (5% vs. 2.5%) of each rejection region for a two tailed test. As a result, the null hypothesis can be rejected with a less extreme result if a one tailed test was used. The one tailed test is only more powerful than a two tailed test if the specified direction of the alternative hypothesis is correct. If it is wrong, however, then the one tailed test has no power.

In specific fields such as particle physics and manufacturing, statistical significance is often expressed in multiples of the standard deviation or sigma (σ) of a normal distribution, with significance thresholds set at a much stricter level (for example 5σ). For instance, the certainty of the Higgs boson particle's existence was based on the 5σ criterion, which corresponds to a p value of about 1 in 3.5 million. In other fields of scientific research such as genome wide association studies, significance levels as low as 5 are not uncommon—as the number of tests performed is extremely large.

Researchers focusing solely on whether their results are statistically significant might report findings that are not substantive and not replicable. There is also a difference between statistical significance and practical significance. A study that is found to be statistically significant may not necessarily be practically significant.

A statistically significant result may not be easy to reproduce.

Starting in the 2010s, some journals began questioning whether significance testing, and particularly using a threshold of α=5%, was being relied on too heavily as the primary measure of validity of a hypothesis. Some journals encouraged authors to do more detailed analysis than just a statistical significance test. In social psychology, the journal Basic and Applied Social Psychology banned the use of significance testing altogether from papers it published, requiring authors to use other measures to evaluate hypotheses and impact. Other editors, commenting on this ban have noted: "Banning the reporting of p values, as Basic and Applied Social Psychology recently did, is not going to solve the problem because it is merely treating a symptom of the problem. There is nothing wrong with hypothesis testing and p values per se as long as authors, reviewers, and action editors use them correctly." Some statisticians prefer to use alternative measures of evidence, such as likelihood ratios or Bayes factors. and may not necessarily improve practice regarding statistical testing. The widespread abuse of statistical significance represents an important topic of research in metascience.

In 2016, the American Statistical Association (ASA) published a statement on p values, saying that "the widespread use of 'statistical significance' (generally interpreted as 'p ≤ 0.05') as a license for making a claim of a scientific finding (or implied truth) leads to considerable distortion of the scientific process". Other researchers responded that imposing a more stringent significance threshold would aggravate problems such as data dredging; alternative propositions are thus to select and justify flexible p value thresholds before collecting data, or to interpret p values as continuous indices, thereby discarding thresholds and statistical significance. Additionally, the change to 0.005 would increase the likelihood of false negatives, whereby the effect being studied is real, but the test fails to show it. In 2019, over 800 statisticians and scientists signed a message calling for the abandonment of the term "statistical significance" in science, and the ASA published a further official statement declaring (page 2): text=We conclude, based on our review of the articles in this special issue and the broader literature, that it is time to stop using the term "statistically significant" entirely. Nor should variants such as "significantly different," "," and "nonsignificant" survive, whether expressed in words, by asterisks in a table, or in some other way.