Mothers against decapentaplegic homolog 3

Protein coding gene in humans

Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It acts as a mediator of the signals initiated by the transforming growth factor beta (TGF β) superfamily of cytokines, which regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.

The human SMAD3 gene is located on chromosome 15 on the cytogenic band at 15q22.33. The gene is composed of 9 exons over 129,339 base pairs. It is one of several human homologues of a gene that was originally discovered in the fruit fly Drosophila melanogaster. The expression of SMAD3 has been related to the mitogen activated protein kinase (MAPK/ERK pathway), particularly to the activity of mitogen activated protein kinase kinase 1 (MEK1). Studies have demonstrated that inhibition of MEK1 activity also inhibits SMAD3 expression in epithelial cells and smooth muscle cells, two cell types highly responsive to TGF β1. The complex of two SMAD3 (or of two SMAD2) and one SMAD4 binds directly to DNA though interactions of the MH1 domain. These complexes are recruited to sites throughout the genome by cell lineage defining transcription factors (LDTFs) that determine the context dependent nature of TGF β action. The DNA binding sites in promoters and enhancers are known as the SMAD binding elements (SBEs). These sites contain the CAG(AC)|(CC) and GGC(GC)|(CG) consensus sequences, the latter also known as 5GC sites. The 5GC motifs are highly represented as clusters of sites, in SMAD bound regions genome wide. These clusters can also contain CAG(AC)|(CC) sites. SMAD3/SMAD4 complex also binds to the TPA responsive gene promoter elements, which have the sequence motif TGAGTCAG. Transcriptional coregulators, such as WWTR1 (TAZ), interact with SMAD3 to promote their function.

The X ray structures of the SMAD3 MH1 domain bound to the GTCT DNA reveal characteristic features of the fold. The MH1 structure consists of four helices and three sets of antiparallel β hairpins, one of which is used to interact with DNA. It also revealed the presence of a bound Zn2+, coordinated by His126, Cys64, Cys109 and Cys121 residues. The MH2 fold is defined by two sets of antiparallel β strands (six and five strands respectively) arranged as a β sandwich flanked by a triple helical bundle on one side and by a set of large loops and a helix on the other.

SMAD3 functions as a transcriptional modulator, binding the TRE (TPA responsive element) in the promoter region of many genes that are regulated by TGF β. SMAD3 and SMAD4 can also form a complex with c Fos and c jun at the AP 1/SMAD site to regulate TGF β inducible transcription. Some of the developmental genes regulated by this pathway include FGF1, NGF, and WNT11 as well as stem/progenitor cell associated genes CD34 and CXCR4. The activity of this pathway as a regulator of pluripotent cell states requires the TRIM33 SMAD2/3 chromatin reading complex. SMAD3 plays also a critical role in TGF β induced repression of target genes, specifically it is required for the repression of c myc. The transcriptional repression of c myc is dependent on direct SMAD3 binding to a repressive SMAD binding element (RSBE), within TIE of the c myc promoter. The c myc TIE is a composite element, composed of an overlapping RSBE and a consensus E2F site, which is capable of binding at least SMAD3, SMAD4, E2F4, and p107. with improved glucose tolerance and insulin sensitivity. Despite their reduced physical activity arising from muscle atrophy, these SMAD3 knockout mice are resistant to high fat diet induced obesity. SMAD3 knockout mouse is a legitimate animal model of human aneurysms‐osteoarthritis syndrome (AOS), also named Loeys Dietz Syndrome (type 3). SMAD3 deficiency promotes inflammatory aortic aneurysms in angiotensin II infused mice via the activation of iNOS. Macrophage depletion and inhibition of iNOS activity prevent aortic aneurysms related to SMAD3 gene mutation

The role of SMAD3 in the regulation of genes important for cell fate, such as differentiation, growth and death, implies that an alteration in its activity or repressing of its activity can lead to the formation or development of cancer. Also several studies have proven the bifunctional tumor suppressor/oncogene role of TGF beta signaling pathway in carcinogenesis. One way in which SMAD3 transcriptional activator function is repressed, is by the activity of EVI 1. EVI 1 encodes a zinc finger protein that may be involved in leukaemic transformation of haematopoietic cells. The zinc finger domain of EVI 1 interacts with SMAD3, thereby suppressing the transcriptional activity of SMAD3. EVI 1 is thought to be able to promote growth and to block differentiation in some cell types by repressing TGF β signalling and antagonizing the growth inhibitory effects of TGF β. The progressive growth of primary tumors and metastases in prostate cancer depends on an adequate blood supply provided by tumor angiogenesis. Studies analyzing SMAD3 levels of expression in prostate cancer cell lines found that the two androgen independent and androgen receptor negative cell lines (PC 3MM2 and DU145) have high expression levels of SMAD3. Analysis of the relation between SMAD3 and the regulation of angiogenic molecules suggest that SMAD3 may be one of key components as a repressor of the critical angiogenesis switch in prostate cancer. The altered activity of SMAD3 was linked to chronic inflammation and somatic mutations that contribute to chronic colitis and the development of colorectal cancer.

TGF β induced SMAD3 transcriptional regulation response has been associated with breast cancer bone metastasis by its effects on tumor angiogenesis, and epithelial mesenchymal transition (EMT). There have been identified diverse molecules that act over the TGF β/SMAD signaling pathway, affecting primarily the SMAD2/3 complex, which have been associated with the development of breast cancer. FOXM1 (forkhead box M1) is a molecule that binds with SMAD3 to sustain activation of the SMAD3/SMAD4 complex in the nucleus. The research over FOXM1 suggested that it prevents the E3 ubiquitin protein ligase transcriptional intermediary factor 1 γ (TIF1γ) from binding SMAD3 and monoubiquitinating SMAD4, which stabilized the SMAD3/SMAD4 complex. FOXM1 is a key player in the activity of the SMAD3/SMAD4 complex, promoting SMAD3 modulator transcriptional activity, and also plays an important role in the turnover of the activity of SMAD3/SMAD4 complex. Based on the importance of this molecule, studies have found that FOXM1 is overexpressed in highly aggressive human breast cancer tissues. The results from these studies also found that the FOXM1/SMAD3 interaction was required for TGF β induced breast cancer invasion, which was the result of SMAD3/SMAD4 dependent upregulation of the transcription factor SLUG. MED15 is a mediator molecule that promotes the activity of the TGF β/SMAD signaling. The deficiency of this molecule attenuates the activity of the TGF β/SMAD signaling pathway over the genes required for induction of epithelial mesenchymal transition. The action of MED15 is related with the phosphorylation of SMAD2/3 complex. The knockdown of MED15 reduces the amount of SMAD3 phosphorylated, therefore reducing its activity as transcription modulator. However, in cancer, MED15 is also highly expressed in clinical breast cancer tissues correlated with hyperactive TGF β signaling, as indicated by SMAD3 phosphorylation. The studies suggest that MED15 increases the metastatic potential of a breast cancer cell line by increasing TGF β induced epithelial–mesenchymal transition.

Smad3 activation plays a role in the pathogenesis of renal fibrosis, probably by inducing activation of bone marrow derived fibroblasts.

The SMAD proteins are homologs of both the Drosophila protein "mothers against decapentaplegic" (MAD) and the C. elegans protein SMA. The name is a combination of the two. During Drosophila research, it was found that a mutation in the gene MAD in the mother repressed the gene decapentaplegic in the embryo. The phrase "Mothers against" was inspired by organizations formed by mothers to oppose social problems, such as Mothers Against Drunk Driving (MADD); and based on a tradition of such unusual naming within the gene research community. A reference assembly of SMAD3 is available.