Циклді нуклеотидтерге сезімтал иондық каналдар

Family of transport proteins
Cyclic nucleotide–gated ion channels or CNG channels are ion channels that function in response to the binding of cyclic nucleotides. CNG channels are nonselective cation channels that are found in the membranes of various tissue and cell types, and are significant in sensory transduction as well as cellular development. Their function can be the result of a combination of the binding of cyclic nucleotides (cGMP and cAMP) and either a depolarization or a hyperpolarization event. Initially discovered in the cells that make up the retina of the eye, CNG channels have been found in many different cell types across both the animal and the plant kingdoms. CNG channels have a very complex structure with various subunits and domains that play a critical role in their function. CNG channels are significant in the function of various sensory pathways including vision and olfaction, as well as in other key cellular functions such as hormone release and chemotaxis. CNG channels have also been found to exist in prokaryotes, including many spirochaeta, though their precise role in bacterial physiology remains unknown.

The discovery of CNG channels is related to the discovery of intracellular messengers responsible for the mediation of responses in retinal photoreceptors. Before their discovery, it was thought that cyclic nucleotides played a role in phosphorylation. In 1985, it was discovered that cGMP was able to directly activate the light dependent response of rod ion channels by studying light adapted retina of frogs. CNG channels were also found in cone photoreceptors, chemo sensitive cilia of olfactory sensory neurons, and the pineal gland. After the identification of amino acids from purified proteins, cloning and functional expression of CNG channels were performed. Molecular cloning allowed for the discovery of similar channels in many other tissues. In 2000, scientists performed studies using mouse retina and molecular cloning to find a new subunit of the channel, CNG6.

CNG channels have important functions in signal transduction in retinal photoreceptors and olfactory receptor neurons. They are directly activated by cyclic nucleotides, and approximately 4 cyclic nucleotides are needed to activate each channel. CNG channels are nonselective and allow many alkali ions to flow into or out of a cell expressing CNG channels on its membrane. This flow of ions can result in either depolarization or hyperpolarization. CNG channels can be activated by cAMP or cGMP exclusively, or sometimes by a combination of both cNMPs, and some channels are more selective than others. Even though the activity of these channels show little voltage dependence, they are still considered voltage dependent channels. Calcium, calmodulin, and phosphorylation modulate the opening of CNG channels.

The structure of the pore is similar to other ion channels that contain P loops. The P loop enters the membrane of the pore from the extracellular side and exits to the intracellular side. The P loop enters as an alpha helix and exists as an uncoiled strand. Helices that cover the inner membrane line the channel. These also form a 6 helix bundle that signifies the entrance. In order to open the pore, a conformational change must occur in the inner 6 helix bundle.

The C linker is a region that connects the CNBD to the S6 segment. The C linker region contributes to the contact between channel subunits as well as promotes tetramerization, the forming of tetramers. There are many residues that play a role in modulation of CNG channels. This process uses metals such as nickel, zinc, copper, and magnesium. The C linker region is involved in the coupling of ligand binding to the opening of the pore. The C linker region forms disulfide bonds with N terminal regions. Disulfide bonds alter the channel function therefore they most likely lie close to the tertiary structure. Disulfide bonds decrease the free energy of the open state compared to the closed state. The specific cysteine residue C481 on the C linker region is located only a few amino acids away from the binding domain. In the closed state C481 is nonreactive; C481 must undergo a conformational change so that it is accessible for the opening of the channel. Disulfide bonds form between neighboring subunits and C481. Simultaneously there is a C35 cysteine residue at the N terminal of the C linker region that can reach two C481 residues, making a favorable disulfide bond compared to a C481 C481 bond. The CNG channels consist of and alpha subunit, known as CNGA1 and a beta subunit CNGB1. An aspect of the alpha subunit is that it is able to form a functional channel on its own, called a homotetrameric channel. This homotetrameric channel can be of scientific interest because it can be used to further understand the nature of ligand binding and selectivity of a particular channel of interest. Additional genes that code for CNG channels have been cloned from Caenorhabditis elegans and Drosophila melanogaster. A subunit of a CNG channel CNGA1, previously called the rod α subunit, was expressed in rod photoreceptors and produced functional channels that were gated by cGMP when expressed externally in either Xenopus oocytes or in a human embryonic kidney cell line (HEK293). In humans, mutated CNGA1 genes result in an autosomal recessive form of retinitis pigmentosa, a degenerative form of blindness. CNGB1, previously called the rod β subunit, is a second subunit of the rod channel. Unlike CNGA1, CNGB1 subunits expressed alone do not produce functional CNG channels, but coexpression of CNGA1 and CNGB1 subunits produces heteromeric channels with modulation, permeation, pharmacology, and cyclic nucleotide specificity comparable to that of native channels. In invertebrates, a CNG channel subunit called CNG P1 has been cloned from D. melanogaster and is expressed in antennae and the visual system, an indication that CNG channels may be linked to the transduction of light in invertebrates. A second putative CNG like subunit called CNGL, cloned from D. melanogaster, is found to be expressed in the brain. Two CNG channel subunits, Tax 2 and Tax 4, have been cloned in C. elegans and are responsible for chemosensation, thermosensation, and normal axon outgrowth of some sensory neurons in C. elegans.

The steep concentration between CNG channels and ligand concentration shows that at least two or three cyclic nucleotides are needed. It is believed that the second ligand is required for the channel to transition from closed to open. When the third and fourth ligands bind, the open state of the channel becomes stabilized.

In the absence of light, cGMP binds to CNG channels in photoreceptors. This binding causes the channels to open, which allows sodium (Na+) and calcium (Ca2+) ions to flow into the cell causing the outer segment of the photoreceptor to depolarize. This depolarizing flow of ions is known as the dark current. When the retina of the eye detects light, a reaction known as a phototransduction cascade occurs. It is a signal transduction pathway that leads to the activation of the enzyme phosphodiesterase, which hydrolyzes cGMP into 5’ GMP, decreasing the concentration of cGMP. In the absence of cGMP, the CNG channels in the photoreceptors close preventing the flow of the aforementioned dark current. This in turn causes a hyperpolarization of the outer segment of the photoreceptor, preventing the propagation of an action potential and the release of glutamate.

Retinitis Pigmentosa (RP) is a genetic disease in which patients suffer degeneration of rod and cone photoreceptors. The loss starts in the patient's peripheral vision and progresses to the central visual field, leaving the patient blind by middle age. About 1% of RP patients have mutations in cGMP alpha subunit. Eight mutations have been identified four are nonsense mutations, one is a deletion that includes most of the transcriptional unit. The other three are missense mutations and frameshift mutations, which lead to a shortening of amino acid sequence in the C terminus. It is still not known why the absence of cGMP gated cation channels causes photoreceptor degradation. Mutations causing RP have also been found in the rhodopsin gene and in the alpha and beta subunits of rod phosphodiesterase, which encode rod phototransduction cascades. The mutation of these subunits indirectly impairs rod cGMP gated channel function, which implies that there is a common mechanism of photoreceptor degradation.

In the nervous system, heart, and some visceral organs, cells contain cyclic nucleotide gated channels which determine the rhythm of the organ. These channels, formally called hyperpolarization activated cyclic nucleotide–gated channels (HCN channels), are also termed "pacemaker channels" because of this critical function. As their name implies, they are open during conditions of hyperpolarization and closed during depolarization. The significance of this in the sinoatrial node (and, as backup, in the atrioventricular node) is that as the heart resets, or hyperpolarizes, after each beat, HCN channels open, allowing positive ions to rush into the cell (the so called funny current), triggering another depolarization event and subsequent cardiac contraction. This gives the heart its automaticity. The primary cyclic nucleotide operating in conjunction with the HCN channel is cAMP.

Almost all responses to odorants in olfactory sensory neurons (OSNs) are facilitated by CNG channels. When an odorant binds to its specific receptor in the chemosensitive cilia membrane, it activates a G protein, which causes a downstream reaction activating the enzyme adenylyl cyclase (AC). This enzyme is responsible for an increase in cAMP concentration within the OSN. cAMP binds to the CNG channels in the OSN membrane, opening them, and making the cell highly permeable to Ca2+. Calcium ions flow into the cell causing a depolarization. As in all other cell types, CNG channels in OSNs also allow Na+ to flow into the cell. Additionally, the increased Ca2+ concentration inside the cell activates Ca2+ dependent chloride (Cl−) channels, which causes intracellular Cl− ions to also flow out of the cell augmenting the depolarization event. This depolarization stimulates an action potential that ultimately signals the reception of the odorant. In addition to cAMP gated ion channels, a small subset of OSNs also has cGMP selective CNG channels that contain the CNGA3 subunit. Differences between retinal and renal cDNA have been implicated in the functional differences between CNG channels in these two tissues. CNG channels play a large role in plant immunity and response to pathogens or external infectious agents. They have also been implicated in apoptosis in plants. CNG ion channels are also thought to be involved in pollen development in plants, however its exact role in this mechanism is still not known. Like mammalian CNG channels, binding of cyclic nucleotides to the CNBDs has been shown to regulate channel activity and alter the channel conformational state. Because these channels were only recently identified in spirochaeta and leptospira species,

Researchers have answered many important questions regarding CNG ion channels functions in vision and olfaction. In other physiological areas, the role of CNG channels is less defined. With technological growth, there now exists more possibilities for understanding these mechanisms. Scientist are speculating whether DAG directly binds with CNG channel during inhibition. It is possible that DAG may insert itself into the transmembrane domains in the channel. It is also possible that DAG inserts itself into the interface between the channel and bilayer. The molecular mechanism of DAG inhibition is still not fully understood.