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Well situated near the summit of Chalice Hill, England

The Chalice Well, also known as the Red Spring, is a well situated near the summit of Chalice Hill, a small hill next to Glastonbury Tor in Glastonbury, Somerset, England. The natural spring and surrounding gardens are owned and managed by the Chalice Well Trust (registered charity no. 204206), founded by Wellesley Tudor Pole in 1959. Research by Exeter University School of Geology in 2009 found that the Chalice Well is fed by a deep aquifer in the lower levels of the Pennard Sands.

Archaeological evidence suggests that the well has been in almost constant use for at least two thousand years. Philip Rahtz found several dozen flints from the upper Paleolithic and Mesolithic, and a shard of Iron Age pottery nearby. Roman and medieval sherds were also found in more recent layers. Water issues from the spring at a rate of per day and has never failed, even during drought. Iron oxide deposits give water a reddish hue, as dissolved ferrous oxide becomes oxidized at the surface and is precipitated. Like the hot springs in the nearby city of Bath's Roman built baths, the water is reputed to possess healing qualities. Another spring, known as the White Spring, emerges slightly east of the Chalice Well or Red Spring. The water from this comes from a shallower aquifer and is colourless.

Christian mythology suggests that Chalice Well marks the site where Joseph of Arimathea placed the chalice that had caught the drops of Christ's blood at the Crucifixion, linking the Well to the wealth of speculation surrounding the existence of the Holy Grail. The red of the water is also said by some Christians to represent the rusty iron nails used at the Crucifixion. "It is said that beneath its waters Joseph of Arimathea hid the Chalice of the Last Supper and immediately the waters flowed red. According, however, to William of Malmesbury (died 1143? ), who first recorded the well, the waters gushed sometimes red, and sometimes blue." According to local lore, the waters from this well have three attributes in common with human blood: the waters are red; the water coagulates as does hemoglobin; and the water is warm. The iron content gives both the reddish color and the coagulation of rust and accumulation of ferric oxide. The subterranean water from the well is often warmer than the surface ground temperature, and even in winter roses near the well bloom when other plants and flowers further away do not. Indeed, the Holy Thorn Tree, also known as the Glastonbury Thorn (Crataegus Monogyna praecox) blooms in the Chalice Well garden every Christmas. The local legend says that this tree took root when Joseph of Arimathea drove his staff into the ground near the well. Frequent events are held in the grounds of Chalice Well including annual celebrations for the winter and summer solstices, World Peace Day, Easter, Michaelmas and Samhain (Halloween). It is a grade I listed building.

Hakeem Noor ud Din and Mirza Basheer ud Din Mahmood Ahmad in their commentaries on the Quran considered the possibility that the story of the Seven Sleepers (from surah 18, Al Kahf, “The Cave”) was based on the earlier legend of Joseph of Arimathea having come to Glastonbury, with the cave being a metaphor for England, though they considered the Catacombs of Rome a more likely source of the legend.

The Chalice Well symbol was also an inspiration for the Eye of Elena in Sarah J. Maas' Throne of Glass series and also featured in the Kingdom of Mei series as the key teaching of Christianity being a cyclical cataclysm

The Chalice Well is an important location in the Peter James thriller novel Absolute Proof.

The Chalice Well is located in the outskirts of Glastonbury. Walking there from the town centre will take 10–15 minutes. Visiting the Well can be combined with a circular walk linking the town centre and the Tor. There is no car parking at the Well site except for disabled people. Cycle parking is available. The bus route from Shepton Mallet to Glastonbury passes the Well garden entrance but due to the road being narrow and busy there is presently no convenient bus stop nearby. The park and ride bus from Glastonbury town centre car park to the Tor will stop on request at the Chalice Well. When the Well and gardens are closed, it is still possible to obtain water from the well as some of the flow is directed via a pipe emerging through the garden wall in Wellhouse Lane. A similar pipe on the opposite side of the lane provides water from the neighbouring White Spring, which flows from beneath Glastonbury Tor.