Тоңқылардың көк құлақ ауруы вирусы және оның тарихы

Species of virus
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Betaarterivirus suid 1, commonly Porcine reproductive and respiratory syndrome virus (PRRSV), is a virus that causes a disease of pigs, called porcine reproductive and respiratory syndrome (PRRS), also known as blue ear pig disease (in Chinese, zhū láněr bìng 豬藍耳病). This economically important, panzootic disease causes reproductive failure in breeding stock and respiratory tract illness in young pigs.

PRRS earlier known as "mystery swine disease" and "blue ear disease" during 1987–1988 in the United States and Canada caused first undiagnosed outbreaks, characterized by reproductive losses combined with respiratory signs. In 1990 1991, a similar disease was reported in several European countries such as Germany, The Netherlands, Belgium, Denmark, France, the United Kingdom, and Spain. The emergence of PRRS in Japan, Taiwan and mainland China was reported in 1987, 1991 and 1996, respectively. According to the WOAH, the disease is now present throughout the world, especially in countries with major pig production. Australia, New Zealand, Norway, Sweden and Switzerland are PRRS free. A better picture of PRRSV's genetic diversity suggested that the virus was present in the field before the "original" outbreaks. The lack of recorded data concerning the prevalence prior to the epidemic was due to unawareness of PRRS. However, still there is an uncertainty as to how, when and where the PRRSV emerged. Several important factors allowed PRRSV to expand further. The transformation of the pig industry worldwide in the 20th century plays a key role. The emergence of high density confinement management practices and adoption of artificial insemination facilitated wide expansion and diversification of PRRSV. Wild animals may act as a natural reservoir for PRRSV and could be considered as an additional source of viral infections in domestic pigs. Highly pathogenic and virulent "atypical" PRRSV isolates are regularly reported in different European countries: Belarus, Belgium, Hungary, Austria and others. Immunization programs of swine herds worldwide with attenuated vaccines made of both PRRSV genotypes provided initially optimistic results in the 1990s. At present PRRS, is one of the most significant infectious disease affecting the swine industry. It was also named as "pig AIDS" for emerging through accidental transspecies transmission and significant economic losses in worldwide pig production.

PRRSV is a member of the family Arteriviridae, and order Nidovirales. Other members of the family Arteriviridae include: equine arteritis virus (EAV), simian hemorrhagic fever virus (SHFV), wobbly possum disease virus, and lactate dehydrogenase elevating virus (LDV).

PRRSV is a small, single stranded, positive sense, enveloped RNA virus. The 15 kb genome consists of one linear, single stranded RNA molecule consisting of a 5′ untranslated region (UTR), ten open reading frames (ORFs 1a 1b 2a 2b 3 4 5 5a 6 7) and a 3′ UTR followed by a polyadenylation tail. Two large ORFs (1a and 1b) comprise 75% of the viral genome. They are translated and processed into 14 non structural proteins including four proteases and the RNA dependent RNA polymerase. A PRRSV particle is composed of eight viral structural proteins encoded by ORFs 2 7. They consist of envelope proteins (GP2a, E, GP3, GP4, GP5, ORF5a and M) and the nucleocapsid (N) protein. To accommodate the clear divergence of the Type 1 and Type 2 PRRSV, they were split into two separate viruses. The genetic variation among the viruses isolated from different places increases the difficulty of developing vaccines against it. Similarly, maintaining diagnostic PCR detection assays is difficult due to the high mutation rate of this virus. In Europe, a geographical demarcation exists between areas of low (Western and Central Europe) and high (Eastern Europe) PRRSV1 diversity. Besides known subtypes, a recent study showed non subtypeable PRRSV1 isolates. It was shown that pigs could be infected with PRRS virus by several routes of exposure: intranasal, intramuscular, peroral, intrauterine, and vaginal. Pigs are most susceptible to infection via intramuscular inoculation (102.2 tissue culture infectious dose with a 50% endpoint (TCID50)), followed by the intranasal route (104.0 TCID50) and peroral route (105.3 TCID50). The basic reproduction number (R0) for Type 1 PRRSV could range from approximately 2 to 5 in naïve pigs. Compared to Type 1 PRRSV isolates, Type 2 PRRSV strains are considered to be of higher virulence. Replication cycle begins with the interaction between the GP5 M complex of PRRSV and the cellular receptors heparan sulfate and CD169 (sialoadhesin). Upon binding and internalization, GP2 GP3 GP4 interacts with CD163 mediating the disassembly and release of the nucleocapsid. After an extensive viremia, the virus reaches macrophages in different internal organs (e. g. lymphoid tissues and maternal endometrium) resulting in disorders, such as reproductive failure in sows. At a late stage of gestation, PRRSV can cross the placental barrier and infect fetuses. Due to these problems, PRRS is considered worldwide as a serious threat for the pig industry. The designation of the term "high pathogenicity" tends to be relative. In general, the highly pathogenic PRRS shows higher reproductive failure rate in pregnant sows, high morbidity and high mortality in pigs of all ages. Infected pigs display a range of respiratory (dyspnea, sneezing, coughing) and systemic clinical signs (a long lasting high fever of over +41 °C, depression, anorexia, discoloration of the skin and the ears), conjunctivitis. Neurological signs could be seen in some cases. The common lesions at necropsy may include inflammation of lungs and lymph nodes, fibrinous pleuropneumonia and pericarditis, peritonitis. Now, quantitative PCR assays offered as good or better sensitivity than nested PCR, fast turnaround time in the lab, and lower rates of cross contamination via closed tube amplification.

Active immunization is currently the only widely available way of PRRS control in swine herds worldwide. For specific immunization of animals against PRRS, several types of vaccines and live virus inoculation are used. The first PRRSV2 live vaccine, Ingelvac PRRS MLV (Boehringer Ingelheim Vetmedica, Inc.), is available for prevention and control of PRRS since 1994. It was obtained by continuous passages in MARC 145 cells. To date, a number of commercial PRRS vaccines have been successfully employed in the global market. The great genetic diversity of PRRSV and the absence of clear immunological parameters correlating with the protection are substantial barriers to new PRRS vaccine development.