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Total mass of fat divided by total body mass, multiplied by 100

The body fat percentage (BFP) of a human or other living being is the total mass of fat divided by total body mass, multiplied by 100; body fat includes essential body fat and storage body fat. Essential body fat is necessary to maintain life and reproductive functions. The percentage of essential body fat for women is greater than that for men, due to the demands of childbearing and other hormonal functions. Storage body fat consists of fat accumulation in adipose tissue, part of which protects internal organs in the chest and abdomen. A number of methods are available for determining body fat percentage, such as measurement with calipers or through the use of bioelectrical impedance analysis. The body fat percentage is a measure of fitness level, since it is the only body measurement which directly calculates a person's relative body composition without regard to height or weight. The widely used body mass index (BMI) provides a measure that allows the comparison of the adiposity of individuals of different heights and weights. While BMI largely increases as adiposity increases, due to differences in body composition, other indicators of body fat give more accurate results; for example, individuals with greater muscle mass or larger bones will have higher BMIs. As such, BMI is a useful indicator of overall fitness for a large group of people, but a poor tool for determining the health of an individual.

Essential fat is the level at which physical and physiological health would be negatively affected, and below which death is certain. Athletic performance might be affected by body fat: A study by the University of Arizona indicated that the ideal body fat percentage for athletic performance is 12–18% for women and 6–15% for men. Bodybuilders may compete at essential body fat range. Certified personal trainers will suggest competitors keep that extremely low level of body fat only for the contest time. However, it is unclear that such levels are ever actually attained since (a) the means to measure such levels are, as noted below, lacking in principle and inaccurate, and (b) 4–6% is generally considered a physiological minimum for human males.

Irrespective of the location from which they are obtained, the fat cells in humans are composed almost entirely of pure triglycerides with an average density of about 0.9 kilograms per litre. Most modern body composition laboratories today use the value of 1.1 kilograms per litre for the density of the "fat free mass". With a well engineered weighing system, body density can be determined with great accuracy by completely submerging a person in water and calculating the volume of the displaced water from the weight of the displaced water. A correction is made for the buoyancy of air in the lungs and other gases in the body spaces. If there were no errors whatsoever in measuring body density, the uncertainty in fat estimation would be about ± 3.8% of the body weight, primarily because of normal variability in body constituents.

Whole body air displacement plethysmography (ADP) is a recognised and scientifically validated densitometric method to measure human body fat percentage. ADP uses the same principles as the gold standard method of underwater weighing, but representing a densitometric method that is based on air displacement rather than on water immersion. Air displacement plethysmography offers several advantages over established reference methods, including a quick, comfortable, automated, noninvasive, and safe measurement process, and accommodation of various subject types (e. g., children, obese, elderly, and disabled persons). However, its accuracy declines at the extremes of body fat percentages, tending to slightly understate the percent body fat in overweight and obese persons (by 1.68–2.94% depending on the method of calculation), and to overstate to a much larger degree the percent body fat in very lean subjects (by an average of 6.8%, with up to a 13% overstatement of the reported body percentage of one individual — i. e. 2% body fat by DXA but 15% by ADP).

A beam of infra red light is transmitted into a biceps. The light is reflected from the underlying muscle and absorbed by the fat. The method is safe, noninvasive, rapid and easy to use.

Dual energy X ray absorptiometry, or DXA (formerly DEXA), is a newer method for estimating body fat percentage, and determining body composition and bone mineral density. X rays of two different energies are used to scan the body, one of which is absorbed more strongly by fat than the other. A computer can subtract one image from the other, and the difference indicates the amount of fat relative to other tissues at each point. A sum over the entire image enables calculation of the overall body composition.

There are several more complicated procedures that more accurately determine body fat percentage. Some, referred to as multicompartment models, can include DXA measurement of bone, plus independent measures of body water (using the dilution principle with isotopically labeled water) and body volume (either by water displacement or air plethysmography). Various other components may be independently measured, such as total body potassium. In vivo neutron activation can quantify all the elements of the body and use mathematical relations among the measured elements in the different components of the body (fat, water, protein, etc.) to develop simultaneous equations to estimate total body composition, including body fat.

There exist various anthropometric methods for estimating body fat. The term anthropometric refers to measurements made of various parameters of the human body, such as circumferences of various body parts or thicknesses of skinfolds. Most of these methods are based on a statistical model. Some measurements are selected, and are applied to a population sample. For each individual in the sample, the method's measurements are recorded, and that individual's body density is also recorded, being determined by, for instance, under water weighing, in combination with a multi compartment body density model. From this data, a formula relating the body measurements to density is developed. Because most anthropometric formulas such as the Durnin Womersley skinfold method, the Jackson Pollock skinfold method, and the US Navy circumference method, actually estimate body density, not body fat percentage, the body fat percentage is obtained by applying a second formula, such as the Siri or Brozek described in the above section on density. Consequently, the body fat percentage calculated from skin folds or other anthropometric methods carries the cumulative error from the application of two separate statistical models. These methods are therefore inferior to a direct measurement of body density and the application of just one formula to estimate body fat percentage. One way to regard these methods is that they trade accuracy for convenience, since it is much more convenient to take a few body measurements than to submerge individuals in water. The chief problem with all statistically derived formulas is that in order to be widely applicable, they must be based on a broad sample of individuals. Yet, that breadth makes them inherently inaccurate. The ideal statistical estimation method for an individual is based on a sample of similar individuals. For instance, a skinfold based body density formula developed from a sample of male collegiate rowers is likely to be much more accurate for estimating the body density of a male collegiate rower than a method developed using a sample of the general population, because the sample is narrowed down by age, sex, physical fitness level, type of sport, and lifestyle factors. On the other hand, such a formula is unsuitable for general use.

The skinfold estimation methods are based on a skinfold test, also known as a pinch test, whereby a pinch of skin is precisely measured by calipers, also known as a plicometer, at several standardized points on the body to determine the subcutaneous fat layer thickness. These measurements are converted to an estimated body fat percentage by an equation. Some formulas require as few as three measurements, others as many as seven. The accuracy of these estimates is more dependent on a person's unique body fat distribution than on the number of sites measured. As well, it is of utmost importance to test in a precise location with a fixed pressure. Although it may not give an accurate reading of real body fat percentage, it is a reliable measure of body composition change over a period of time, provided the test is carried out by the same person with the same technique. Skinfold based body fat estimation is sensitive to the type of caliper used, and technique. This method also only measures one type of fat: subcutaneous adipose tissue (fat under the skin). Two individuals might have nearly identical measurements at all of the skin fold sites, yet differ greatly in their body fat levels due to differences in other body fat deposits such as visceral adipose tissue: fat in the abdominal cavity. Some models partially address this problem by including age as a variable in the statistics and the resulting formula. Older individuals are found to have a lower body density for the same skinfold measurements, which is assumed to signify a higher body fat percentage. However, older, highly athletic individuals might not fit this assumption, causing the formulas to underestimate their body density.

Ultrasound is used extensively to measure tissue structure and has proven to be an accurate technique to measure subcutaneous fat thickness. A mode and B mode ultrasound systems are now used and both rely on using tabulated values of tissue sound speed and automated signal analysis to determine fat thickness. By making thickness measurements at multiple sites on the body you can calculate the estimated body fat percentage. Ultrasound techniques can also be used to directly measure muscle thickness and quantify intramuscular fat. Ultrasound equipment is expensive, and not cost effective solely for body fat measurement, but where equipment is available, as in hospitals, the extra cost for the capability to measure body fat is minimal. For males, they measure the neck and waist just above the navel. Females are measured around the hips, waist, and neck. These measurements are then looked up in published tables, with the individual's height as an additional parameter. This method is used because it is a cheap and convenient way to implement a body fat test throughout an entire service. Methods using circumference have little acceptance outside the Department of Defense due to their negative reputation in comparison to other methods. The method's accuracy becomes an issue when comparing people with different body compositions, those with larger necks artificially generate lower body fat percentage calculations than those with smaller necks.