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Mist based plant growing process

Aeroponics is the process of cultivating plants in an air or mist environment, eliminating the need for soil or an aggregate medium. The term "aeroponic" originates from the ancient Greek: aer (air) and ponos (labor, hardship, or toil). It falls under the category of hydroponics, as water is employed in aeroponics to deliver nutrients to the plants.

The fundamental principle of aeroponic growing entails suspending plants in a closed or semi closed environment whilst spraying their dangling roots and lower stems with a nutrient rich water solution in an atomized or sprayed form. The upper portion of the plant, including the leaves and crown, referred to as the canopy, extends above. The plant support structure keeps the roots separated. To minimize labor and expenses, closed cell foam is often compressed around the lower stem and inserted into an opening in the aeroponic chamber. In the case of larger plants, trellising is employed to support the weight of the vegetation and fruits. The goal is to maintain an environment free from pests and diseases, allowing the plants to thrive and grow faster than those cultivated in a growing medium. However, since most aeroponic environments are not completely sealed off from the outside, pests and diseases can still pose a threat. Controlled environments facilitate the advancement of plant development, health, growth, flowering, and fruiting for various plant species and cultivars. Due to the sensitivity of root systems, aeroponics is often combined with conventional hydroponics. This serves as a backup nutrition and water supply in case of any failure in the aeroponic system, acting as an emergency "crop saver." High pressure aeroponics refers to the method of delivering nutrients to the roots using mist heads with a size range of 20 50 micrometers. This is achieved using a high pressure diaphragm pump operating at around 80 pounds per square inch (550 kPa).

Air cultures maximize air exposure to facilitate optimal plant growth. The materials and devices that hold and support aeroponically grown plants must be completely free from disease or pathogens. A vital characteristic of a genuine aeroponic culture and apparatus is minimal plant support features. This design allows maximum airflow around the plant by minimizing contact between the plant and the support structure. In long term aeroponic cultivation, it is crucial to ensure that the root systems are unconstrained, allowing unrestricted growth, root expansion, unhindered access to pure water, adequate air exchange, and disease free conditions. The isolating nature of aeroponic systems enables researchers to avoid complications encountered when studying these infections in soil cultures.

Aeroponic equipment employs sprayers, misters, foggers, or other devices to create a fine mist of solution for delivering nutrients to plant roots. Aeroponic systems are typically closed looped systems designed to provide macro and micro environments that sustain reliable and consistent air cultures. Several inventions have been developed to facilitate aeroponic spraying and misting. The size of the water droplet is critical for root development in an aeroponic environment. In commercial applications, a 360° hydro atomizing spray is used, which utilizes air pressure misting to cover large areas of roots. A variation of the mist technique, known as fogponics, utilizes ultrasonic foggers to mist nutrient solutions in low pressure aeroponic devices. Water droplet size plays a vital role in maintaining aeroponic growth. Water droplets that are too large can limit the availability of oxygen to the root system. Conversely, excessively fine water droplets generated by ultrasonic misters can lead to excessive root hair growth without developing a lateral root system necessary for sustained growth in an aeroponic system. In their research, the team found that by measuring the concentrations and volumes of input and efflux solutions, they could accurately calculate the nutrient uptake rate. To validate their findings, they compared the results with N isotope measurements. Once their analytical method was verified, Barak et al. proceeded to gather additional data specific to cranberries. This included studying diurnal variations in nutrient uptake, examining the correlation between ammonium uptake and proton efflux, and exploring the relationship between ion concentration and uptake. These findings highlight the potential of aeroponics not only as a valuable research tool for studying nutrient uptake but also as a means to monitor plant health and optimize crop cultivation in closed environments. Atomization at pressures exceeding 65 pounds per square inch (450 kPa) increases the bioavailability of nutrients. Consequently, nutrient strength must be significantly reduced to prevent leaf and root burn. It's worth noting the large water droplets in the photo on the right, which indicate that the feed cycle may be too long or the pause cycle too short. Both scenarios discourage lateral root growth and root hair development. Optimal results are achieved when feed cycles are as short as possible, with roots remaining slightly damp but never excessively dry. A typical feed/pause cycle is less than 2 seconds of feeding followed by approximately 1.5 2 minutes of pause, maintained continuously. However, when an accumulator system is incorporated, cycle times can be further reduced to less than approximately 1 second of feeding and around 1 minute of pause.

Shortly after its development, aeroponics emerged as a valuable research tool, providing researchers with a noninvasive method to examine developing roots. This innovative technology expanded the possibilities for conducting experiments by offering a larger number of parameters and a wider range of experimental conditions. The precise control over root zone moisture levels and water delivery makes aeroponics particularly well suited for studying water stress. K. Hubick evaluated aeroponics as a means to consistently produce plants with minimal water stress, which can be utilized in drought or flood physiology experiments. Aeroponics stands out as the ideal tool for investigating root morphology. The absence of aggregates enables researchers to easily access the entire, intact root structure without causing damage that may occur when removing roots from soils or aggregates. It has been observed that aeroponics yields more natural root systems compared to hydroponics.

Aeroponic cultivation involves growing plants in an air culture, allowing them to develop and grow naturally.

In 1911, Арциховский, Владимир Мартынович published an article titled "On Air Plant Cultures" in the journal "Experienced Agronomy." In this article, he introduced his method of conducting physiological studies on root systems by spraying various substances in the surrounding air, which is now known as the aeroponics method. Artsikhovski designed the first aeroponic systems and demonstrated their effectiveness for plant cultivation. In 1942, W. Carter conducted pioneering research on air culture growing and described a method for growing plants in water vapor to facilitate root examination. Since 2006, aeroponics has been widely used in agriculture worldwide. In 1944, L. J. Klotz made an important discovery by misting citrus plants, which facilitated his research on diseases affecting citrus and avocado roots. In 1952, G. F. Trowel successfully grew apple trees using a spray culture technique. GTi's device featured an open loop water driven system controlled by a microchip. It delivered a high pressure hydro atomized nutrient spray within an aeroponic chamber. The Genesis Machine was designed to be connected to a water faucet and an electrical outlet, providing the necessary resources for operation. as well as enhanced root and overall growth due to nutrient supply through the aeroponic system (Santos and Fisher, 2009). Additionally, the absence of rooting media reduces the risk of root diseases (Mehandru et al., 2014). Aeroponics plays a crucial role in propagating plants that have low success rates in vegetative propagation, plants with significant medicinal uses, high demand plants, and in creating new cultivars of specific plant species. For instance, Leptadenia reticulata, an important medicinal plant with low reproductive rates through both seeds and cuttings,

Aeroponics serves as a more advantageous alternative to the traditional method of using overhead misters (Peterson et al., 2018). It boasts a higher success rate compared to overhead misters, which have drawbacks such as the need for large volumes of water, potential unsanitary conditions, uneven misting coverage, and possible leaching of foliar nutrients (Peterson et al., 2018). In 1982, L. Nir in Israel developed a patent for an aeroponic apparatus that utilized compressed low pressure air to deliver a nutrient solution to suspended plants held by styrofoam inside large metal containers. In 1976, British researcher John Prewer conducted a series of aeroponic experiments in the UK, where lettuces were grown from seed to maturity in 22 days using polyethylene film tubes. The fog droplets used in these experiments were generated by equipment supplied by Mee Industries of California. In collaboration with John Prewer, a commercial grower named Kings Nurseries on the Isle of Wight used a different design of aeroponics system in 1984 to grow strawberry plants. The strawberries flourished, yielding a bountiful crop that was highly appreciated by customers, especially the elderly, who valued the cleanliness, quality, and flavor of the fruit, as well as the convenience of picking it without stooping. In 1983, R. Stoner filed a patent for the first microprocessor interface designed to deliver tap water and nutrients into an enclosed aeroponic chamber made of plastic. Stoner subsequently established several companies dedicated to researching and advancing aeroponic hardware, interfaces, biocontrols, and components for commercial aeroponic crop production. In the 1990s, General Hydroponics [Europe] (GHE) attempted to introduce aeroponics to the hobby hydroponics market and introduced the Aerogarden system. Although the Aerogarden did not meet the criteria of "true" aeroponics as it produced droplets instead of a fine mist, it created a demand for aeroponic growing in the hobby market. The distinction between mist aeroponics and droplet aeroponics became blurred in the eyes of many. However, a UK firm called Nutriculture conducted trials of true mist aeroponics, which showed positive results compared to traditional growing techniques like Nutrient Film Technique (NFT) and Ebb & Flood. Despite the drawbacks of cost and maintenance, Nutriculture developed a scalable, easy to use droplet aeroponic system called the Amazon, acknowledging that better results could be achieved by propagating plants in their branded X stream aeroponic propagator and then transferring them to the specially designed droplet aeroponic growing system.

In 1986, Stoner achieved a significant milestone by becoming the first person to successfully market fresh aeroponically grown food to a national grocery chain. His accomplishment garnered attention, and he was invited for an interview on NPR, where he highlighted the significance of aeroponics in terms of water conservation, both in modern agriculture and even in space exploration. The Sputnik 4 mission involved carrying seeds of wheat, pea, maize, spring onion, and Nigella damascena, while Chlorella pyrenoidosa cells were taken into orbit on the Discoverer 17 mission. Following these early endeavors, plant experiments were conducted on various missions involving Bangladesh, China, and joint Soviet American efforts, including Biosatellite II, Skylab 3 and 4, Apollo Soyuz, Sputnik, Vostok, and Zond. Initial research findings shed light on how low gravity affected the orientation of roots and shoots (Halstead and Scott, 1990).

In 1996, Richard Stoner's research on organic disease control (ODC) received funding from NASA. The goal was to develop a natural liquid biocontrol solution for closed loop hydroponic systems that could prevent plant diseases and increase yields without the need for pesticides. By 1997, NASA conducted biocontrol experiments with Stoner's ODC solution. The experiments took place using BioServe Space Technologies' GAP technology, which consisted of miniature growth chambers. Bean seeds were treated with the ODC solution in triplicate experiments conducted aboard the MIR space station, at the Kennedy Space Center, and at Colorado State University under total darkness conditions to eliminate light as a variable. The focus of the NASA experiment was solely on studying the benefits of the biocontrol. Results from NASA's enclosed environment bean experiments on the MIR space station and shuttle confirmed that ODC promoted increased germination rates, better sprouting, enhanced growth, and activated natural plant disease mechanisms. Although initially developed for NASA, ODC is not limited to space applications. Soil and hydroponics growers can also incorporate ODC into their planting techniques, as it complies with USDA NOP standards for organic farming. One notable example of ODC's expansion in agriculture is its application in the cannabis industry. The ODC product line has been developed specifically for emerging agricultural crops like cannabis. The active ingredients in the ODC cannabis line include the original chitosan ingredient at a concentration of 0.25%, as well as 0.28% colloidal nitrogen and 0.05% calcium. In order to enhance the resilience of hydroponic and aeroponic systems against plant diseases and reduce reliance on chemical additives, NASA explores the integration of environmental biocontrols into the design of these systems. The Advanced Plant Habitat (APA), deployed on the International Space Station (ISS) since 2018, exemplifies this approach. Equipped with over 180 sensors, the APA optimizes plant growth and health while decreasing the need for chemical additive biocontrols. The sensors monitor various environmental factors, including lighting intensity, spectrum, and photoperiod, temperature, CO2 levels, relative humidity, irrigation, as well as plant derived ethylene and volatile organic compound (VOC) scrubbing. Additionally, the APA features leaf and root zone temperature sensors, root zone moisture sensors, and oxygen concentration meters. These environmental controls serve two main purposes in inhibiting plant diseases. Firstly, they maintain environmental conditions that directly hinder the growth of diseases, fungi, and pests. By carefully regulating factors like temperature and humidity, the risk of infections, such as botrytis in leaves, is reduced as the environment becomes less conducive to disease proliferation. Secondly, these controls create conditions that promote the plant's natural disease prevention mechanisms, indirectly inhibiting the effects of plant diseases. For instance, experiments with peppers conducted under blue light conditions have shown increased resilience to powdery mildew.

In 1998, Stoner received funding from NASA to develop a high performance aeroponic system suitable for use on Earth as well as in space. Through his research, Stoner demonstrated significant increases in growth rates of lettuce plants cultivated in aeroponic systems compared to other cultivation techniques. NASA subsequently adopted many of the aeroponic advancements developed by Stoner. Research efforts have focused on identifying and developing technologies for rapid plant growth in different gravitational environments. Low gravity environments present challenges such as efficient delivery of water and nutrients to plants, as well as the recovery of waste products. Food production in space also requires addressing issues like water management, minimizing water usage, and reducing system weight. Additionally, future food production on planetary bodies like the Moon and Mars will involve dealing with reduced gravity environments. Given the varying fluid dynamics at different levels of gravity, optimizing nutrient delivery systems has been a major focus in developing plant growth systems. Various nutrient delivery methods are currently employed, both on Earth and in low gravity environments. Substrate dependent methods include traditional soil cultivation, zeoponics, agar, and nutrient loaded ion exchange resins. In addition to substrate dependent approaches, non soil methods have been developed, including the nutrient film technique, ebb and flow, aeroponics, and others. Hydroponic systems, with their high nutrient solution throughput, can achieve rapid plant growth. However, this necessitates large volumes of water and significant recycling of the solution, which poses challenges for controlling solutions in microgravity conditions. Aeroponic systems use hydro atomized sprays to deliver nutrients, resulting in minimal water usage, enhanced root oxygenation, and excellent plant growth. The nutrient solution throughput of aeroponic systems is higher compared to other systems designed for low gravity environments. Aeroponics eliminates the need for substrates and reduces the quantity of waste material that must be managed by other life support systems. By removing the substrate requirement, planting and harvesting processes are simplified, automation becomes easier, the weight and volume of expendable materials are reduced, and a potential pathway for pathogen transmission is eliminated. These advantages highlight the potential of aeroponic production in microgravity environments and its efficiency in food production for outer space. The AIS is a self contained and self supporting system designed to deliver nutrients and mist to plant roots in an aeroponic environment. Its inflatable structure offers the advantage of being lightweight and can be deflated to occupy less volume during transportation and storage. This current version of AIS represents an improvement over previous designs that utilized rigid structures, which tend to be more costly to manufacture and transport.

Aeroponics possesses numerous characteristics that contribute to its effectiveness and efficiency as a method of plant cultivation.

Plants grown using aeroponics spend 99.98% of their time in the air, with only 0.02% in direct contact with hydro atomized nutrient solution. This minimal contact with water allows the roots to efficiently capture oxygen. Additionally, the hydro atomized mist plays a significant role in effectively oxygenating the roots. A comparison between aeroponics and Nutrient Film Technique (NFT) reveals that aeroponics has a lower nutrient throughput of 1.5 milliliters per minute, in contrast to NFT's 1 liter per minute. The reduced volume of nutrient throughput in aeroponics leads to a reduction in the overall amount of nutrients required for plant development. Another advantage of the reduced throughput, particularly relevant for space applications, is the decreased water volume used. This reduction in water volume not only lightens the weight needed to sustain plant growth but also reduces the buffer volume. Additionally, the volume of effluent produced by the plants is minimized in aeroponics, resulting in a reduced amount of water that requires treatment for reuse. The use of relatively small solution volumes in aeroponics, combined with the limited exposure of roots to the hydro atomized mist, minimizes root to root contact and reduces the spread of pathogens among plants.

Aeroponics provides greater control over the environment surrounding the root zone compared to other plant growth systems like hydroponics. In aeroponics, the plant roots are not continuously surrounded by any medium.

Aeroponics offers the flexibility to administer various nutrient solutions to the root zone without the need to flush out previous solutions or matrices. This high level of control is particularly valuable for studying the impact of different nutrient regimens on specific plant species. Moreover, aeroponics enables a wider range of growth conditions compared to other nutrient delivery systems. For instance, the interval and duration of nutrient sprays can be finely adjusted to meet the specific requirements of a plant species. This means that the aboveground tissue can experience a different environment from that of the roots.

The design of an aeroponic system enables convenient handling of plants. This is due to the individual separation of plants and their suspension in the air, free from any matrix that could entrap the roots. As a result, harvesting each plant becomes a straightforward task. Similarly, removing any infected plant is easily done without the risk of uprooting or contaminating nearby plants.

Aeroponic systems offer cost effective advantages compared to other systems. The reduced volume of solution throughput, as mentioned earlier, translates to lower water and nutrient requirements in the system. Additionally, the elimination of substrates and the need for many moving parts contribute to cost savings.

Aeroponics offers a solution to minimize the negative impact of pathogens in seed stocks. As previously mentioned, the separation of plants and the absence of a shared growth matrix contribute to this advantage. Moreover, aeroponics provides an enclosed and controlled environment, making it an ideal system for growing pathogen free seed stocks. The enclosed growth chamber, combined with the isolation of plants from each other, serves to prevent initial contamination from external pathogens and limits the spread of any existing pathogens among plants.

Aeroponics represents a significant advancement in artificial life support for plants, offering benefits such as non damaging plant support, efficient seed germination, precise environmental control, and unrestricted growth. In comparison to traditional agricultural techniques like hydroponics and drip irrigation, which have been in use for decades, aeroponics provides notable improvements in plant cultivation.

Contemporary aeroponic techniques have been extensively researched at NASA's BioServe Space Technologies ( ) located on the campus of the University of Colorado in Boulder, Colorado. This research center focuses on the development and commercialization of aeroponic systems. Additionally, scientists at Ames Research Center have conducted research on enclosed loop systems, investigating methods for growing food crops in low gravity environments to support future space colonization efforts. In 2000, Stoner was granted a patent for his organic disease control biocontrol technology, which enables pesticide free cultivation in aeroponic systems. A notable milestone in aeroponics occurred in 2004 when Ed Harwood, the founder of AeroFarms, invented an innovative aeroponic system that utilizes micro fleece cloth to grow lettuces. AeroFarms, leveraging Harwood's patented aeroponic technology, currently operates the largest indoor vertical farm in the world based on its annual growing capacity in Newark, New Jersey. This state of the art farm employs aeroponic technology to produce and distribute up to two million pounds of pesticide free leafy greens each year.

Aeroponic bio pharming is an innovative approach used to cultivate pharmaceutical medicines within plants. This technology provides complete containment, ensuring that effluents and by products of biopharma crops are confined within a closed loop facility. In a notable development in 2005, Dr. Neil Reese of South Dakota State University conducted GMO research using aeroponics to grow genetically modified corn. Dr. Reese considers it a significant achievement to successfully grow corn in an aeroponic system for bio massing. Previous attempts at growing various types of corn using hydroponics had been unsuccessful. Through the implementation of advanced aeroponics techniques, Dr. Reese was able to harvest mature ears of genetically modified corn while effectively containing the corn pollen and spent effluent water, thus preventing their release into the environment. This containment ensures that the surrounding environment remains free from GMO contamination. Dr. Reese emphasizes that aeroponics offers the potential for economically viable bio pharming practices, making it a promising avenue for pharmaceutical production. The integration of aeroponics in Vietnamese agriculture begins with the production of low cost, certified disease free organic minitubers. These minitubers then serve as a local supply for farmers engaged in field plantings of seed potatoes and commercial potatoes. The adoption of aeroponics will benefit potato farmers by providing them with disease free seed potatoes grown without the use of pesticides. Importantly, it will also reduce their operational costs and increase their yields, according to Thach.