Протромбиназа кешені: Протромбиннің тромбинге айналуын катализдеуші факторлар.

Complex that catalyzes conversion of prothrombin to thrombin
The prothrombinase enzyme complex consists of factor Xa (a serine protease) and factor Va (a protein cofactor). The complex assembles on negatively charged phospholipid membranes in the presence of calcium ions. The prothrombinase complex catalyzes the conversion of prothrombin (factor II), an inactive zymogen, to thrombin (factor IIa), an active serine protease. The activation of thrombin is a critical reaction in the coagulation cascade, which functions to regulate hemostasis in the body. To produce thrombin, the prothrombinase complex cleaves two peptide bonds in prothrombin, one after Arg271 and the other after Arg320. Although it has been shown that factor Xa can activate prothrombin when unassociated with the prothrombinase complex, the rate of thrombin formation is severely decreased under such circumstances. The prothrombinase complex can catalyze the activation of prothrombin at a rate 3 x 105 fold faster than can factor Xa alone. Thus, the prothrombinase complex is required for the efficient production of activated thrombin and also for adequate hemostasis.

Both factor X and factor V circulate in the blood as inactive precursors prior to activation by the coagulation cascade. The inactive zymogen factor X consists of two chains, a light chain (136 residues) and a heavy chain (306 residues). The light chain contains an N terminal γ carboxyglutamic acid domain (Gla domain) and two epidermal growth factor like domains (EGF1 and EGF2). The heavy chain consists of an N terminal activation peptide and a serine protease domain. Factor X can be activated by both the factor VIIa tissue factor complex of the extrinsic coagulation pathway and by the tenase complex of the intrinsic pathway. The intrinsic tenase complex is composed of both factor IXa and factor VIIIa. The activation peptide is released when factor X is activated to factor Xa, but the heavy and light chains remain covalently linked following activation. Factor V circulates as a single chain procofactor which contains six domains, A1 A2 B A3 C1 C2. Thrombin activates factor V by cleaving off the B domain. Other proteases also activate factor V, but this cleavage is primarily carried out by thrombin. Following cleavage, factor Va contains a heavy chain, composed of the A1 and A2 domains and a light chain, consisting of the A3, C1, and C2 domains. The light and heavy chains of factor Va are linked via a divalent metal ion, such as calcium.

Prothrombinase assembly begins with the binding of factor Xa and factor Va to negatively charged phospholipids on plasma membranes. Activated factor Xa and factor Va bind to the plasma membranes of a variety of different cell types, including monocytes, platelets, and endothelial cells. Both factor Xa and Va bind to the membrane independently of each other, but they both bind to mutually exclusive binding sites. Both factor Xa and factor Va associate with the membrane via their light chains, with factor Xa binding via its Gla domain in a calcium dependent manner and factor Va via its C2 and C1 domains. Once bound to the plasma membrane, Factor Xa and factor Va rapidly associate in a 1:1 stoichiometric ratio to form the prothrombinase complex. Assembly of the prothrombinase complex is calcium dependent. When associated with the prothrombinase complex, the catalytic efficiency of factor Xa is increased 300,000 fold compared to its efficiency alone. Factor Va also decreases the Km of the reaction by enhancing the binding of prothrombin to the prothrombinase complex.

The fully assembled prothrombinase complex catalyzes the conversion of the zymogen prothrombin to the serine protease thrombin. Specifically, Factor Xa cleaves prothrombin in two locations, following Arg271 and Arg320 in human prothrombin. Meizothrombin contains fragment 1•2 A chain linked to the B chain by a disulfide bond. Subsequent cleavage of meizothrombin by factor Xa at Arg271 gives fragment 1•2 and active thrombin, consisting of the A and B chains linked by a disulfide bond. When thrombin is generated by factor Xa alone, the first pathway predominates and prothrombin is first cleaved after Arg271, producing prethrombin 2, which is subsequently cleaved after Arg320. If factor Xa acts as a component of the prothrombinase complex, however, the second pathway is favored, and prothrombin is first cleaved after Arg320, producing meizothrombin, which is cleaved after Arg271 to produce active thrombin. Thus, the formation of the prothrombinase complex alters the sequence of prothrombin bond cleavage.

Factor Va is inactivated following cleavage by activated protein C. Activated protein C cleaves factor Va in both its light and heavy chains. Cleavage in the heavy chain reduces the ability of factor V to bind to factor Xa. Activated protein C interacts tightly and exclusively with the light chain of factor Va, and this interaction is calcium independent. Factor Xa can help to prevent the inactivation of factor Va by protecting factor Va from activated protein C. It is likely that factor Xa and activated protein C compete for similar sites on factor Va. Congenital factor X deficiency is also extremely rare, affecting an estimated 1 in 1,000,000. A point mutation in the gene encoding factor V can lead to a hypercoagulability disorder called factor V Leiden. In factor V Leiden, a G1691A nucleotide replacement results in an R506Q amino acid mutation. Factor V Leiden increases the risk of venous thrombosis by two known mechanisms. First, activated protein C normally inactivates factor Va by cleaving the cofactor at Arg306, Arg506, and Arg679. The factor V Leiden mutation at Arg506 renders factor Va resistant to inactivation by activated protein C. As a result of this resistance, the half life of factor Va in plasma is increased, resulting in increased thrombin production and increased risk of thrombosis. Secondly, under normal conditions, if factor V is cleaved by activated protein C instead of thrombin, it can serve as a cofactor for activated protein C.

Heterozygous factor V Leiden is present in approximately 5% of the white population in the United States and homozygous factor V Leiden is found less than 1% of this population. Factor V Leiden is much more common in individuals of Northern European descent and in some Middle Eastern populations. It is less common in Hispanic populations, and rare in African, Asian, and Native American populations. In fact, heterozygous factor V Leiden increases one's risk of recurrent venous thromboembolism by 40%.

Inhibition of factor Xa prevents thrombin activation, thereby preventing clot formation. Thus, Factor Xa is used as both a direct and indirect target of several anticoagulant drugs. For example, the drug Fondaparinux is an indirect inhibitor of factor Xa. Fondaparinux binds to antithrombin III and activates the molecule for factor Xa inhibition. In fact, Fondaparinux imparts an increased affinity of antithrombin III to factor Xa, and this increased affinity results in a 300 fold increase in the antithrombin III inhibitory effect on factor Xa. After the antithrombin III binds to factor Xa, the Fondaparinux is released and can activate another antithrombin. Another drug that indirectly inhibits factor Xa is Idraparinux. Idraparinux also binds antithrombin III, however with a 30 fold increase in affinity as compared to Fondaparinux. Idraparinux has an increased half life as compared to Fondaparinux and can be administered on a weekly basis, whereas Fondaparinux must be subcutaneously injected daily. Rivaroxaban, Apixaban and Edoxaban are direct factor Xa inhibitors. Rivaroxaban, Apixaban and Edoxaban bind to the active site of factor Xa, regardless of whether factor Xa is bound in the prothrombinase complex or is in its free form. These direct factor Xa inhibitors can be administered orally, as can dabigatran etexilate, which is a direct thrombin inhibitor. Fondaparinux, Rivaroxaban, Apixaban, Dabigatran Etexilate and Edoxaban are currently used as FDA approved anticoagulant drugs. Development of Idraparinux was discontinued.