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In music theory, a perfect fifth is the musical interval corresponding to a pair of pitches with a frequency ratio of 3:2, or very nearly so. In classical music from Western culture, a fifth is the interval from the first to the last of the first five consecutive notes in a diatonic scale. The perfect fifth (often abbreviated P5) spans seven semitones, while the diminished fifth spans six and the augmented fifth spans eight semitones. For example, the interval from C to G is a perfect fifth, as the note G lies seven semitones above C.

The perfect fifth may be derived from the harmonic series as the interval between the second and third harmonics. In a diatonic scale, the dominant note is a perfect fifth above the tonic note. The perfect fifth is more consonant, or stable, than any other interval except the unison and the octave. It occurs above the root of all major and minor chords (triads) and their extensions. Until the late 19th century, it was often referred to by one of its Greek names, diapente. Its inversion is the perfect fourth. The octave of the fifth is the twelfth. A perfect fifth is at the start of "Twinkle, Twinkle, Little Star"; the pitch of the first "twinkle" is the root note and the pitch of the second "twinkle" is a perfect fifth above it.

The term perfect identifies the perfect fifth as belonging to the group of perfect intervals (including the unison, perfect fourth and octave), so called because of their simple pitch relationships and their high degree of consonance. When an instrument with only twelve notes to an octave (such as the piano) is tuned using Pythagorean tuning, one of the twelve fifths (the wolf fifth) sounds severely discordant and can hardly be qualified as "perfect", if this term is interpreted as "highly consonant". However, when using correct enharmonic spelling, the wolf fifth in Pythagorean tuning or meantone temperament is actually not a perfect fifth but a diminished sixth (for instance G–E). Perfect intervals are also defined as those natural intervals whose inversions are also natural, where natural, as opposed to altered, designates those intervals between a base note and another note in the major diatonic scale starting at that base note (for example, the intervals from C to C, D, E, F, G, A, B, C, with no sharps or flats); this definition leads to the perfect intervals being only the unison, fourth, fifth, and octave, without appealing to degrees of consonance. The term perfect has also been used as a synonym of just, to distinguish intervals tuned to ratios of small integers from those that are "tempered" or "imperfect" in various other tuning systems, such as equal temperament. The perfect unison has a pitch ratio 1:1, the perfect octave 2:1, the perfect fourth 4:3, and the perfect fifth 3:2. Within this definition, other intervals may also be called perfect, for example a perfect third (5:4) or a perfect major sixth (5:3).

In addition to perfect, there are two other kinds, or qualities, of fifths: the diminished fifth, which is one chromatic semitone smaller, and the augmented fifth, which is one chromatic semitone larger. In terms of semitones, these are equivalent to the tritone (or augmented fourth), and the minor sixth, respectively.

[[File:Just perfect fifth below A. png|thumb|Just perfect fifth below A. The perfect fifth below A (D , 10/9) is a syntonic comma lower than the just/Pythagorean major second above middle C: (D, 9/8). meaning that the upper note makes three vibrations in the same amount of time that the lower note makes two. The just perfect fifth can be heard when a violin is tuned: if adjacent strings are adjusted to the exact ratio of 3:2, the result is a smooth and consonant sound, and the violin sounds in tune. Keyboard instruments such as the piano normally use an equal tempered version of the perfect fifth, enabling the instrument to play in all keys. In 12 tone equal temperament, the frequencies of the tempered perfect fifth are in the ratio or approximately 1.498307. An equally tempered perfect fifth, defined as 700 cents, is about two cents narrower than a just perfect fifth, which is approximately 701.955 cents. Kepler explored musical tuning in terms of integer ratios, and defined a "lower imperfect fifth" as a 40:27 pitch ratio, and a "greater imperfect fifth" as a 243:160 pitch ratio. His lower perfect fifth ratio of 1.48148 (680 cents) is much more "imperfect" than the equal temperament tuning (700 cents) of 1.4983 (relative to the ideal 1.50). Hermann von Helmholtz uses the ratio 301:200 (708 cents) as an example of an imperfect fifth; he contrasts the ratio of a fifth in equal temperament (700 cents) with a "perfect fifth" (3:2), and discusses the audibility of the beats that result from such an "imperfect" tuning.

W. E. Heathcote describes the octave as representing the prime unity within the triad, a higher unity produced from the successive process: "first Octave, then Fifth, then Third, which is the union of the two former". Hermann von Helmholtz argues that some intervals, namely the perfect fourth, fifth, and octave, "are found in all the musical scales known", though the editor of the English translation of his book notes the fourth and fifth may be interchangeable or indeterminate. The perfect fifth is a basic element in the construction of major and minor triads, and their extensions. Because these chords occur frequently in much music, the perfect fifth occurs just as often. However, since many instruments contain a perfect fifth as an overtone, it is not unusual to omit the fifth of a chord (especially in root position). The perfect fifth is also present in seventh chords as well as "tall tertian" harmonies (harmonies consisting of more than four tones stacked in thirds above the root). The presence of a perfect fifth can in fact soften the dissonant intervals of these chords, as in the major seventh chord in which the dissonance of a major seventh is softened by the presence of two perfect fifths. Chords can also be built by stacking fifths, yielding quintal harmonies. Such harmonies are present in more modern music, such as the music of Paul Hindemith. This harmony also appears in Stravinsky's The Rite of Spring in the "Dance of the Adolescents" where four C trumpets, a piccolo trumpet, and one horn play a five tone B flat quintal chord.

The just perfect fifth, together with the octave, forms the basis of Pythagorean tuning. A slightly narrowed perfect fifth is likewise the basis for meantone tuning. The circle of fifths is a model of pitch space for the chromatic scale (chromatic circle), which considers nearness as the number of perfect fifths required to get from one note to another, rather than chromatic adjacency.